Actin-Toolkit Fluorescence Microscopy
ATTO532-labeled native α-actin from rabbit skeletal muscle
Cat. #: 8085-01
Applications
- Visualization of ligand-induced actin polymerization.
- Microscopic analysis of actin filament bundles and networks.
Description
| Product name | Actin-Toolkit Fluorescence Microscopy | ATTO532-Actin |
| Protein | ATTO532-Actin (α-actin) |
| Origin | Rabbit skeletal muscle |
| Molecular mass | 42 kDa |
| Toolkit description |
The Actin-Toolkit Fluorescence Microscopy is designed to analyze ligand-induced actin polymerization and the formation of actin filament bundles or networks under defined in vitro conditions. ATTO532-Actin can be combined with actin-interacting ligands to visualize effects on G-actin or F-actin by fluorescence microscopy. The kit additionally contains unlabeled rabbit skeletal muscle α-actin, allowing labeled and unlabeled actin to be mixed when a lower fluorescent fraction is desired. Vinculin tail, a V8-protease cleavage product of vinculin, is supplied as an actin-bundling reference. MonoMix, PolyMix and MgCl2 are included for preparation and polymerization of actin under controlled conditions. A dedicated handbook provides step-by-step experimental setups for fluorescence microscopy with labeled actin. |
| Optical properties | ATTO532-Actin: excitation 532 nm, emission 553 nm |
| Purity | >99% by scanning densitometry. |
Properties
| Form | Lyophilized. |
| Kit content | 4 × 100 µg ATTO532-Actin (lyophilized powder); 1 × 0.5 mg unlabeled α-skeletal muscle actin (lyophilized powder); 1 × 50 µg Vinculin tail; 5 × 50 ml MonoMix (lyophilized G-actin ATP buffer); 1 × 1.0 ml PolyMix, 10× stock (lyophilized F-actin ATP buffer, pH 7.4); 1 × 0.5 ml 1 M MgCl2; 1 × Toolkit Handbook Fluorescence Microscopy; 10 × light-protective tubes and tags. |
| Actin buffer after reconstitution | 2 mM Tris-Cl, pH 8.2, 0.4 mM ATP, 0.5 mM DTT, 0.1 mM CaCl2 and 0.3% disaccharides when reconstituted with 1.0 ml ultrapure water to obtain a 1 mg/ml actin solution. |
| Related products | Actin-binding proteins and additional fluorescent actin conjugates for defined in vitro imaging assays. |
| Purification notes | Native actin purified from rabbit skeletal muscle; final purification by gel permeation chromatography (GPC). ATTO532-Actin is prepared from purified actin. |
| Protein concentration | Actin concentration is determined by direct UV absorbance at A290 nm with correction for fluorophore absorbance where applicable. For unlabeled actin, A290nm 1% = 0.62. |
| Storage instructions | We guarantee stable performance of the kit components for 6 months when stored at −70 °C upon arrival. Solubilized G-actin should be kept on ice and used within 1 week. Avoid refreezing. |
| Shipping conditions | Shipped at ambient temperature. Upon delivery store at −70 °C. |
| CAS no. | 51005-14-2 (Actin) |
| Remarks | For research use only. Not for diagnostic or therapeutic use. |
Further Information
Handbook Actin-Toolkit Fluorescence Microscopy
(Web version only for demonstration purpose!)
A full print version with protocols come with the kit.
Flyer Actin-Toolkits
Material and Safety Data Sheet
Protein Sequence on NCBI
