Actin-Toolkits


Detection and Analyses of Actin Interactions
Ready-to-use for instant, reliable results!

 

Actin-Toolkits are designed for a whole spectrum of applications. Ranging from the verification of the biological activity of a potential actin binding protein to comprehensive sets of assays characterizing its biochemical or structural properties.

Actin-Toolkits are all-in-one applications for:

  •  fail-safe handling of actin assays,
  •  saving your time and 
  •  maximizing your research efforts.

 

‘Tool a protein’

Actin-Toolkits utilize actin as a tool for the functional & structural analysis of ligands with actin binding sites. Actin-Toolkits are based on established in vitro and in situ methods. They are also convenient to use for experienced researchers who wish to design their own methods.


  
The Collection of Actin-Toolkits

Actin-Toolkits apply to certain methods analyzing and characterizing ligand-actin interactions. Guided by the handbook coming with each kit, a stepwise introduction to the use of actin for the chosen application is given. Experienced researchers designing their own experimental setups may choose between the convenient usage of Actin-Toolkits or individual proteins and components from our assortment.

 

 


Product categories:  

 

Actin-Toolkit F-Actin Binding (alpha skeletal muscle actin)
  • Characterization and analysis of F-actin binding proteins interacting with alpha-skeletal muscle actin.
  • Ready-to-use. 
447,00 EUR
 
Actin-Toolkit G-Actin Binding (alpha skeletal muscle actin)
TOP
  • Proof of ligands biological activity by G-actin binding;
  • Functional analysis of ligands binding to G-actin;
  • Analysis of pharmacological compounds, proteins or lipids altering
  • ligand-G-actin-interaction;
382,00 EUR
 
Actin-Tookit Fluorometry (alpha skeletal muscle alpha actin, pyrene-actin)
  • Kinetic analysis of purified ligands, cell or tissue extracts on polymerization or depolymerization of actin;

  • Determination of sensitivity to divalent cations or pharmacological compounds during polymerization or depolymerization; 

  • Measurement of F-actin severing effects of purified ligands, cell or tissue extracts at steady-state;

827,00 EUR
 
Actin-Toolkit Ligand Interaction
  • Fluorescence analysis of native G-actin complexes;
  • Works with crude cell or tissue extracts;
  • A direct quick step assay without using antibodies to proof actin-ligand interactions; 
 
 
302,00 EUR
 
Actin-Toolkit Crystallography

 

 

  • Co-crystallization of actin binding proteins with Non-Polymerizable Actin;
  • Stabilization of ligands during crystallization by complex formation;
  • Analysis of conformational changes upon actin binding;
  • Suitable for biochemical applications;
1.420,00 EUR
 
Actin-Toolkit Microinjection (Rhodamine-Actin, skeletal muscle actin)
  • Visualization of transfected actin binding proteins effecting the actin cytoskeleton;
  • Study of the effects by injection of pre-formed ligand-actin complexes;
  • Analysis of components or culturing conditions on the actin cytoskeleton;
  • Suitable for use with cell or tissue extracts in biochemical assays;
394,00 EUR
 
Actin-Toolkit ELISA (alpha skeletal muscle actin)
  • Designed for direct and Sandwich ELISA, to analyse and quantify G- or F-actin ligand interactions with alpha-skeletal muscle actin. 
  • Ready-to-use.
358,00 EUR
 
Actin-Toolkit Microinjection (ATTO565-Actin, skeletal muscle actin)
  • Visualization of transfected actin binding proteins effecting the actin cytoskeleton;
  • Study of the effects by injection of pre-formed ligand-actin complexes;
  • Analysis of components or culturing conditions on the actin cytoskeleton;
  • Suitable for use with cell or tissue extracts in biochemical assays;
427,00 EUR
 
Actin-Toolkit SPR (alpha skeletal muscle actin)
  • Kinetic and affinity analysis of F-actin binding ligands, specifically binding to α-skeletal muscle actin.
  • Ready-to-use.
488,00 EUR
 
Actin-Toolkit G-Actin Binding (alpha cardiac actin)
  • Proof of ligands biological activity by G-actin binding;
  • Functional analysis of ligands binding to G-actin;
  • Analysis of pharmacological compounds, proteins or lipids altering ligand-G-actin-interaction;
478,00 EUR
 
Actin-Toolkit F-Actin Binding (alpha cardiac actin)
  • Characterization and analysis of F-actin binding proteins interacting with alpha-cardiac actin.
  • Ready-to-use. 
553,00 EUR
 
Actin-Toolkit Microinjection (bovine alpha-cardiac actin)
  • Visualization of transfected actin binding proteins effecting the actin cytoskeleton;
  • Study of the effects by injection of pre-formed ligand-actin complexes;
  • Analysis of components or culturing conditions on the actin cytoskeleton;
  • Suitable for use with cell or tissue extracts in biochemical assays;
520,00 EUR
 
Actin-Toolkit ELISA (alpha cardiac actin)
  • Designed for direct and Sandwich ELISA, to analyse and quantify G- or F-actin ligand interactions with alpha-skeletal muscle actin. 
     
  • Ready-to-use.
504,00 EUR
 
Actin-Toolkit SPR (alpha cardiac muscle actin)
  • Kinetic and affinity analysis of F-actin binding ligands, specifically binding to α-cardiac muscle actin.
  • Ready-to-use.
525,00 EUR
 
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