ATPase Pi (ATPase Assay)
ATPase Pi
Kit for determination of ATPase activities.
Description
| Product name | ATPase Pi ATPase Assay |
| Assay principle | ATPase Pi is used to determine enzymatic ATPase activity by quantifying inorganic phosphate released during ATP hydrolysis. |
| Product description | ATPase Pi is suited for ATPase activity measurements of myosins, S1, HMM and actomyosin. The assay operates in a moderate inorganic phosphate detection range of 50 µM to 1 mM, supporting robust measurement of ATPase activities where highly sensitive phosphate assays may be less suitable. The assay is based on a modified Fiske–Subbarow method. Under reducing conditions, inorganic phosphate forms a blue phosphomolybdate complex. Organic phosphates remain unrecognized, making the assay compatible with phosphoproteins and nucleotides. |
| Overview | Reproducible results; stable components; easy handling. |
Properties
| Form | Powder. Ready-to-use upon addition of the specified components. |
| Components | Solution M: 1 × 100 ml molybdate solution; add 100 ml 2.6 N sulphuric acid. Solution R: 1 × 100 ml reduction solution; add 100 ml ultrapure H2O. Phosphate standard: 50 ml 1 mM KPO4, ready-to-use. Standardized solutions in ultrapure H2O: 1 M imidazole pH 7.0, 3 M KCl, 0.1 M MgCl2, 0.1 M CaCl2, 0.1 M EDTA pH 7.0 and 0.1 M ATP pH 7.0. |
| Additional component required | TCA / trichloroacetic acid, 50% solution, 2.6 N sulphuric acid. |
| Quantity | Approximately 2000 assays. |
| Safety note | Dangerous liquids are not shipped with this kit. Required components must be added in accordance with applicable laboratory safety instructions. MSDS documents for substances to be added are available on request. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
