ATPase Pi (ATPase Assay)
ATPase Pi
Kit for determination of ATPase activities.
Cat. #: 6061-01
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Description
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Product name |
ATPase Pi (ATPase Assay) |
Product description |
The ATPase Pi is used to determine the enzymatic activity of e.g. myosins, S1, HMM and actomyosin, by measuring the inorganic phosphate released upon ATP-hydrolysis |
Overview |
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Properties
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Form |
Powder. Ready-to-use upon addition of components (see below). |
Components
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1. Solution M – 1x 100ml Molybdate Solution (add 100ml 2.6N sulphuric acid)
2. Solution R – 1x100ml Reduction Solution (add 100ml H2O ultrapure)
3. Phosphate-Standard (50ml 1mM KPO4 – ready-to-use)
4. Standardized solutions in H2O ultrapure: 1M imidazole pH 7.0, 3M KCl, 0.1M
MgCl2, 0.1M CaCl2, 0.1M EDTA pH 7.0, 0.1M ATP pH 7.0
Additional components required: TCA- trichloroacetic acid (50% solution)
Please note that we do not ship dangerous liquids. Thus these components are to be added following the laboratory safety instructions to protect your health. You are welcome to contact us for submission of the MSDSs for the substances to be added. |
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