Rabbit Anti-Mouse IgG (H+L)
Antibody produced in Rabbit, affinity purified
Cat. #: 3123-2MG
Applications
Unconjugated anti-mouse IgG (H+L) suitable for immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF) after user conjugation, and general immunoassays. The purified, carrier-free format supports custom labeling with fluorophores, enzymes, biotin, or other tags, and can be used as a capture or detection reagent in ELISA- and bead-based workflows where defined antibody composition is required.
Description
| Product name | Rabbit Anti-Mouse IgG (H+L) | RXM |
| Target species | Mouse |
| Description |
Rabbit Anti-Mouse IgG (H+L) is an unconjugated secondary antibody that binds mouse primary antibodies by recognizing both heavy and light chains (H+L). It is commonly used for immunohistochemistry and a broad range of immunoassay formats where reliable detection of mouse IgG is required. The antibody is supplied affinity purified to reduce non-specific rabbit serum proteins and to support low-background staining. The carrier-free, unconjugated format is well suited for user-directed labeling (e.g., fluorochromes, enzymes, biotin), enabling customization for multiplex detection panels and assay-specific signal development. In addition to use as a secondary detection reagent, Rabbit Anti-Mouse IgG (H+L) can be employed as a capture antibody in enzyme immunoassays or in other analytical platforms requiring purified immunoglobulins with minimal additives. |
| Host | Rabbit |
| Specification | Rabbit Anti-Mouse IgG (H+L), lyophilized |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) |
| Content | 2 mg Rabbit Anti-Mouse IgG (H+L) Buffer: 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Column affinity purified. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
| CAS no. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet 
Material and Safety Data SheetReferences
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
Product DataSheet
Material and Safety Data Sheet
Table of Dyes and Light Sources
Catalogue: ATTO Secondary Antibodies
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
