Swinholide - 100µM
Swinholide A
Cell-permeant actin-disrupting marine macrolide for F-actin severing and actin dimer stabilization.
Applications
| Applications | Cell-permeant marine natural compound for rapid disruption of the actin cytoskeleton. Swinholide A is used to depolymerize F-actin, sever actin filaments and stabilize actin dimers in biochemical and cell-based cytoskeleton assays. |
| Recommended use | Recommended for experiments requiring fast F-actin disassembly, collapse of pre-existing actin networks, actin filament fragmentation or mechanistic comparison of actin-targeting compounds. |
| Typical workflows | Used in cell-based and biochemical assays to study actin filament turnover, cytoskeletal collapse, F-actin stability, actin depolymerization, microfilament disruption and the functional comparison of actin-targeting natural compounds. |
Description
| Product name | Swinholide A |
| Product description | Swinholide A is a bioactive dimeric dilactone macrolide isolated from the marine sponge Theonella swinhoei. It is a potent actin-disrupting compound that binds actin, stabilizes actin dimers and severs F-actin filaments. Swinholide A is used as a pharmacological research tool to collapse actin filament networks and to study actin filament stability, depolymerization and cytoskeletal remodeling. Its dual activity makes it especially useful when rapid fragmentation of filamentous actin or disruption of already assembled actin structures is required. |
| Mechanism | Swinholide A disrupts the actin cytoskeleton by a dual mechanism: direct F-actin severing and stabilization of actin dimers. This distinguishes it from compounds that primarily prevent polymerization by sequestering monomeric actin. |
| Comparison to Latrunculin A | Latrunculin A mainly blocks actin polymerization by sequestering monomeric G-actin. Swinholide A is more directly directed against filament integrity because it severs F-actin and stabilizes actin dimers. |
| Overview | Cell-permeant actin-disrupting marine macrolide; induces actin depolymerization; severs actin filaments; stabilizes actin dimers; suitable for rapid disruption of actin cytoskeletal structures. |
Properties
| Form | Lyophilized, ready-to-use after reconstitution in DMSO. |
| Stock solution | Add 25 µl DMSO to obtain a 100 µM Swinholide A stock solution. |
| Calculated amount | 100 µM in 25 µl corresponds to 2.5 nmol Swinholide A. |
| Chemical class | Dimeric dilactone macrolide; marine natural compound. |
| Source | Theonella swinhoei. |
| Formula | C78H132O20 |
| Molecular weight | 1389.9 g/mol |
| CAS no. | 95927-67-6 |
| Solubility | Soluble in DMSO; also reported soluble in ethanol and methanol. |
| Biological activity | Cell-permeant F-actin inhibitor. Swinholide A induces actin depolymerization by severing actin filaments and stabilizing actin dimers. |
| Storage instructions | Store at −20 °C upon arrival. Protect from light and moisture. Avoid repeated freeze-thaw cycles after reconstitution. |
| Shipping conditions | At ambient temperature. Upon delivery, store at −20 °C. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
Compounds and reagents from Hypermol® are prepared for reliable research use, as described in our product documentation.
References
Bubb MR et al. Swinholide A is a microfilament disrupting marine toxin that stabilizes actin dimers and severs actin filaments. J Biol Chem. 1995;270(8):3463-3466.
Saito S et al. Actin-depolymerizing effect of dimeric macrolides, bistheonellide A and swinholide A. J Biochem. 1998;123(4):571-578.
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Compounds and reagents from Hypermol® are prepared for reliable research use, as described in our product documentation.
Compounds and reagents from Hypermol® are prepared for reliable research use, as described in our product documentation.