MotorMix I (for Myosin II filaments) - 100ml
MotorMix I
Buffer for use with myosin II filaments, S1 or HMM
Cat. #: 5502-01
Description
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Description
MotorMix I is physiological buffer for myosin II filaments (thick filaments) and to make myosin filaments from myosin II solutions. The buffer is also vital for myosin S1 or heavy meromyosin (HMM). DTT and ATP are provided with the buffer to be freshly added before use.
The MotorMix I BufferKit consists of three components: MotorMix I, DTT and ATP. This allows preparation of two buffer variants used for myosin II filaments, Myosin S1 and HMM.
The first variant is to supplement MotorMix I with 2mM DTT. This step is essential to prevent oxidation of myosin SH-groups. DTT is always added freshly to the MotorMix I, due its short half-life time.
The second variant is used to initiate the myosin ATPase by addition of e.g. 1mM ATP. ATP can be added either to the MotorMix I/DTT solution or mixed with the protein in MotorMix I/DTT.
The MotorMix I BufferKit consists of three components: MotorMix I, DTT and ATP. This allows preparation of two buffer variants used for myosin II filaments, Myosin S1 and HMM.
The first variant is to supplement MotorMix I with 2mM DTT. This step is essential to prevent oxidation of myosin SH-groups. DTT is always added freshly to the MotorMix I, due its short half-life time.
The second variant is used to initiate the myosin ATPase by addition of e.g. 1mM ATP. ATP can be added either to the MotorMix I/DTT solution or mixed with the protein in MotorMix I/DTT.
Content
100 ml MotorMix I (solution)
1 ml 100mM ATP (powder)
0.5 ml 500mM DTT (powder)
Further Information
Product DataSheet
Material and Safety Data Sheet
Buffers from Hypermol are made of purest reagents in Milli-Q™ water, as described in our publications.
1 ml 100mM ATP (powder)
0.5 ml 500mM DTT (powder)
Further Information
Product DataSheetMaterial and Safety Data SheetBuffers from Hypermol are made of purest reagents in Milli-Q™ water, as described in our publications.
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