Atto647-Actin for TIRFM
α-actin from skeletal muscle, rabbit
Cat. #: 8158-03
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Description
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Protein |
Atto647-Actin |
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Origin |
Skeletal muscle alpha actin, rabbit |
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Molecular mass |
42kDa |
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Protein description |
Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. ATTO647-Actin is chemically modified G-actin with an ATTO647 fluorescent group covalently bound to lysine residues. |
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Conjugation ratio |
This product is supplied with a degree of labelling (DOL) of ~0.3, suited for TIRF microscopy. |
Properties
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Form |
Lyophilized, ready-to-use. |
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Quantity per unit |
2x100 µg |
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Buffer |
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100µl ultrapure H2O to obtain a 1mg/ml solution. |
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Purity |
>99% by scanning densitometry. |
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Purification notes |
Purified from rabbit skeletal muscle. |
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Protein concentration |
Determined by the Biuret method. |
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Storage instructions |
Atto647-Actin stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized product is kept on ice, protect from light and it should be used within 1 week.Avoid refreezing of actin. |
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Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
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Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
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CAS no. |
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Further Information
Product DataSheet
Material and Safety Data Sheet
Table of Dyes and Light Sources
Protein Sequence on NCBI
References
Phosphoinositides regulate force-independent interactions between talin, vinculin, and actin.
Kelley CF, Litschel T, Schumacher S, Dedden D, Schwille P, Mizuno N. , Elife. 2020;9:e56110. Published 2020 Jul 13. doi:10.7554/eLife.56110
Kank2 activates talin, reduces force transduction across integrins and induces central adhesion formation.
Sun Z, Tseng HY, Tan S, Senger F, Kurzawa L, Dedden D, Mizuno N, Wasik AA, Thery M, Dunn AR, Fässler R.
Nat Cell Biol. 2016 Sep;18(9):941-53. doi: 10.1038/ncb3402. Epub 2016 Aug 22.
Product DataSheet
Material and Safety Data Sheet
Table of Dyes and Light Sources
Protein Sequence on NCBI
Phosphoinositides regulate force-independent interactions between talin, vinculin, and actin.
Kelley CF, Litschel T, Schumacher S, Dedden D, Schwille P, Mizuno N. , Elife. 2020;9:e56110. Published 2020 Jul 13. doi:10.7554/eLife.56110
Kank2 activates talin, reduces force transduction across integrins and induces central adhesion formation.
Sun Z, Tseng HY, Tan S, Senger F, Kurzawa L, Dedden D, Mizuno N, Wasik AA, Thery M, Dunn AR, Fässler R.
Nat Cell Biol. 2016 Sep;18(9):941-53. doi: 10.1038/ncb3402. Epub 2016 Aug 22.
