ATTO532-Actin (alpha-cardiac actin, bovine) - 2x100µg
ATTO532-Actin
Fluorescent alpha-cardiac actin from bovine cardiac muscle; lysine-labelled with ATTO532.
Applications
| Applications | Fluorescent, polymerization-competent alpha-cardiac actin for fluorescence microscopy, FRET, fluorescence spectroscopy, filament tracking and biochemical actin assays. |
Description
| Product name | ATTO532-Actin |
| Protein | Alpha-cardiac actin, bovine. |
| Molecular mass | 42 kDa. |
| Product description | ATTO532-Actin is chemically modified bovine cardiac G-actin carrying covalently attached ATTO532 fluorescent groups on lysine residues. The protein is prepared from alpha-cardiac actin isolated from bovine cardiac muscle. Cardiac actin is a single-chain polypeptide of approximately 42 kDa consisting of 375 amino acids. ATTO532-Actin is designed for fluorescence-based actin assays and provides a defined fluorescent signal while retaining the polymerization properties required for actin-based biochemical workflows. |
| Fluorophore | ATTO532. |
| Spectral properties | Excitation maximum: 532 nm; emission maximum: 553 nm. |
| Labelling chemistry | ATTO532 is covalently attached to lysine residues of cardiac G-actin. |
| Degree of labelling | This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8. |
| Overview | Fluorescent bovine cardiac G-actin; ATTO532-labelled on lysine residues; polymerization-competent; suitable for fluorescence microscopy, FRET, fluorescence spectroscopy and biochemical actin assays; supplied lyophilized in actin-compatible buffer. |
Properties
| Form | Lyophilized, ready-to-use after reconstitution. |
| Quantity per unit | 2 × 100 µg. |
| Reconstitution | Reconstitute each 100 µg tube with 100 µl ultrapure water to obtain a 1 mg/ml ATTO532-Actin solution. |
| Buffer | 2 mM Tris-HCl pH 8.2, 0.4 mM ATP, 0.5 mM DTT, 0.1 mM CaCl2 and 0.3% disaccharides after reconstitution with ultrapure water to obtain a 1 mg/ml solution. |
| Purity | >99% by scanning densitometry. |
| Purification notes | Purified by GPC from bovine cardiac muscle. |
| Protein concentration | Determined by UV absorbance at 290 nm (A290 = 0.63 for 1 mg/ml actin) with fluorophore-specific correction factor applied. |
| Storage instructions | ATTO-Actins stored at −70 °C upon arrival will be stable in performance for at least 6 months from the date of purchase. Solubilized ATTO-Actin should be kept on ice and used within 1 week. Avoid refreezing. |
| Shipping conditions | At ambient temperature. Upon delivery store at −70 °C. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
| CAS no. | |
Proteins and buffers from Hypermol® are prepared with ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Download product datasheet for ATTO532-Actin.
Material and Safety Data Sheet
MSDS / SDS document for ATTO-labelled cardiac muscle actin products.
Protein Sequence on NCBI
Reference sequence information for bovine cardiac alpha-actin.
Hide ATTO dyes & spectral properties
Open by default; click to collapse. Hover/tap a dye for excitation hints.
ATTO dyes – spectral overview (λex / λem)
Hover/tap a dye to see details incl. possible excitation source.
References
Kank2 activates talin, reduces force transduction across integrins and induces central adhesion formation.
Sun Z, Tseng HY, Tan S, Senger F, Kurzawa L, Dedden D, Mizuno N, Wasik AA, Thery M, Dunn AR, Fässler R.
Nat Cell Biol. 2016 Sep;18(9):941-953. doi: 10.1038/ncb3402.
