ATTO532-Actin (alpha-cardiac actin, bovine) - 2x100µg

Protein

ATTO532-Actin

Origin

Alpha cardiac actin, bovine

Molecular mass

42kDa

Protein description

Actin from bovine cardiac muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. ATTO532-Actin is chemically modified cardiac G-actin with an ATTO532 fluorescent group covalently bound to lysine residues. ATTO532-Actin (λex 532nm, λem 553nm) possesses the polymerization properties of native G-actin. 

Conjugation ratio

This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8. The precise DOL is stated in the DataSheet supplied with the product.

Form

Lyophilized, ready-to-use.

Quantity per unit

2x100 µg

Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100 µl ultrapure water to obtain a 1mg/ml solution.

Purity

>99% by scanning densitometry.

Purification notes

Purified by GPC from bovine cardiac muscle.

Protein concentration

Determined by the Biuret method.

Storage instructions

ATTO-Actins are stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized ATTO-Actin is kept on ice and should be used within 1 week. Avoid refreezing.  

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

CAS no.