Actin-Toolkit Electron Microscopy (bovine alpha-cardiac actin)

Protein

Actin (alpha-cardiac actin)

Origin

Cardiac muscle, bovine

Molecular mass

42kDa

Toolkit description

Electron microscopic analysis of negatively stained proteins is a highly evident method to demonstrate the effects of ligands on actin. The Actin-Toolkit Electron Microscopy is ready-to use for the preparation of electron microscopic specimen, making this method accessible for researchers who would like to get introduced to EM works on actin imaging.

This Toolkit is designed for users that either took a short introduction at an EM facility or to pass kit and ligands for inspection to an EM service. The Actin-Toolkit Electron Microscopy is easy-to-use. It contains 4x0.5mg rabbit skeletal (Cat. # 8070-01) or bovine cardiac muscle actin (Cat. # 8700-01), buffers for washing the specimen that maintain either the G- or F-actin state, and SpreadingSolution in addition. 

A handbook with a detailed description of the experimental setup guides the user for safe, error-free handling. We guarantee excellent performance of kit components when stored at -70°C upon arrival for 6 months.

Purity

>99% by scanning densitometry.

Form

Lyophilized, ready-to-use.

Kit content

4x0.25mg G-Actin (lyophilized powder);
4x50ml MonoMix (lyophilized G-actin ATP-buffer);
2x1.0ml PolyMix 10x stock (lyophilized F-actin ATP-buffer, pH 7.4);
1x0.5ml SpreadingSolution;
1x0.5ml 1M MgCl2;
4x Centrifuge-Minifilter (0.2µm);
1x Toolkit Handbook Electron Microscopy;

Actin Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100µl ultrapure water to obtain a 1mg/ml solution.

Purity

>99% (according to scanning densitometry);

Purification notes

Purified from bovine cardiac muscle, GPC.

DOL

Determined by the Biuret method.

Storage instructions

We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing.     

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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