Actin-Toolkit Electron Microscopy
Native bovine cardiac α-actin
Cat. #: 8700-01
Applications
- TEM analysis of ligands bound to monomeric actin, actin filaments, bundles or networks.
- Determination of actin filament length, thickness and structural properties.
Description
| Product name | Actin-Toolkit Electron Microscopy | Bovine Cardiac Actin |
| Protein | Actin (cardiac α-actin) |
| Origin | Bovine cardiac muscle |
| Molecular mass | 42 kDa |
| Toolkit description |
The Actin-Toolkit Electron Microscopy is designed for transmission electron microscopy of negatively stained actin specimens. It supports structural analysis of ligand interactions with monomeric actin and with filamentous actin structures such as individual filaments, bundles and networks. The toolkit contains native bovine cardiac α-actin together with buffers for maintaining G-actin or F-actin conditions during specimen preparation. SpreadingSolution and 0.2 µm centrifuge minifilters are included for preparation of EM specimens. A dedicated handbook describes the experimental setup and specimen-preparation workflow for electron microscopic analysis of actin. |
| Purity | >99% by scanning densitometry. |
Properties
| Form | Actin and buffer components are supplied lyophilized where indicated; liquid components are supplied as listed below. |
| Kit content | 4 × 0.25 mg G-Actin (lyophilized powder); 4 × 50 ml MonoMix (lyophilized G-actin ATP buffer); 2 × 1.0 ml PolyMix, 10× stock (lyophilized F-actin ATP buffer, pH 7.4); 1 × 0.5 ml SpreadingSolution; 1 × 0.5 ml 1 M MgCl2; 4 × centrifuge minifilters (0.2 µm); 1 × Toolkit Handbook Electron Microscopy. |
| Actin buffer after reconstitution | 2 mM Tris-Cl, pH 8.2, 0.4 mM ATP, 0.5 mM DTT, 0.1 mM CaCl2 and 0.3% disaccharides. Reconstitute 0.25 mg actin with 250 µl ultrapure water to obtain 1 mg/ml. |
| Purification notes | Purified from bovine cardiac muscle; GPC. |
| Protein concentration | Actin concentration determined by direct UV absorbance at 290 nm; A290 nm, 1% = 0.62. |
| Storage instructions | Store at −70 °C upon arrival. Kit components are stable for 6 months under these conditions. Solubilized G-actin should be kept on ice and used within 1 week. Avoid refreezing. |
| Shipping conditions | Shipped at ambient temperature. Upon delivery store at −70 °C. |
| Remarks | For research use only. Not for human or veterinary diagnostic or therapeutic applications. |
Further Information
Handbook Actin-Toolkit Electronmicroscopy
(Web version only for demonstration purpose!)
A full print version with protocols come with the kit.
Flyer Actin-Toolkits
Material and Safety Data Sheet
Protein Sequence on NCBI