PolyMix 8 (actin polymerization buffer, 1ml -10x)

Product name

PolyMix 8

Product description

PolyMix 8 is used to polymerize G-actin solutions at pH 6.0, dilute F-actin or actin ligands. The buffer contains all components essential for the biological activity of actin. 

Overview

PolyMix 8 is used to:

• facilitate ligand binding to F-actin at pH 8.0;
• polymerize G-actin to F-actin;
• support the F-actin state;
• prevent denaturing of actin;

Form

Lyophilized, ready-to-use.

Quantity

1x1.0 ml 10x stock

Buffer

Powder to make 1.0ml of a 10x actin polymerization buffer. Including 1M KCl, 10mM ATP, 20mM MgCl2. Buffer: Imidazole pH 5.0.
The buffer is added1:10 v/v to actin solutions.

Purity

Ultrapure reagents.

Storage instructions

PolyMix 8 is stored at -70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Avoid refreezing.    

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

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