Product

ActinStain ATTO490LS

Origin

Amanita Phalloides Conjugate

Molecular mass

N/A

Product description

Rapid and selective staining of single actin filaments with highly photostable ActinStain ATTO490LS is based on the selective affinity of phalloidin for filamentous actin. Phalloidin is a bicyclic peptide of a family of toxins isolated from the Amanita phalloides “death cap” mushroom. The peptide is commonly used as a selective stain or counterstain to specifically label F-actin in fixed or permeabilized cells, and cell-free experiments. Labeled phalloidin conjugates bind in a stoichiometric ratio of about one phallotoxin per actin subunit in both muscle and non-muscle cells, and have similar affinity for both large and small filaments. Phalloidins do not bind to monomeric actin and have a stabilizing effect on F-actin. 

ActinStain ATTO490LS is a novel dye due to its large stokes shift of 165nm. It is excited with green light and emits red light fluorescence of unparalleled brightness and photostability.

Actin interaction

Selective staining of actin filaments in vitro.

Form

Dry

Quantity

1x 100 Units (sufficient for 100 slides)

Buffer

Reconstitute in 100µl MeOH, DMSO or DMF. Dilute 5µl of the phalloidin stock with 500µl PBS and apply to stain the sample.

Sub cellular localization

Actin, Cytoskeleton

Excitation/Emission

ex 496 nm, em 661 nm

Kit Content

1x 100 Units ActinStain ATTO490LS;
1x PolyMix (10x conc., F-actin buffer, lyophilized); 

Storage instructions

Upon reconstitution the product is stored at -5 to -70°C. Always protect from light.

Shipping conditions 

At ambient temperature. Upon delivery store at -5 to -70°C.

Remarks

CAS no.

 N/A