ActinStain ATTO 490LS
Cat. #: 8816-01
Applications
Selective, rapid staining of single actin filaments with highly photostable ActinStain ATTO490.
Description
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|
Product |
ActinStain ATTO490LS |
Origin |
Amanita Phalloides Conjugate |
Molecular mass |
N/A |
Product description |
Rapid and selective staining of single actin filaments with highly photostable ActinStain ATTO490LS is based on the selective affinity of phalloidin for filamentous actin. Phalloidin is a bicyclic peptide of a family of toxins isolated from the Amanita phalloides “death cap” mushroom. The peptide is commonly used as a selective stain or counterstain to specifically label F-actin in fixed or permeabilized cells, and cell-free experiments. Labeled phalloidin conjugates bind in a stoichiometric ratio of about one phallotoxin per actin subunit in both muscle and non-muscle cells, and have similar affinity for both large and small filaments. Phalloidins do not bind to monomeric actin and have a stabilizing effect on F-actin.
ActinStain ATTO490LS is a novel dye due to its large stokes shift of 165nm. It is excited with green light and emits red light fluorescence of unparalleled brightness and photostability. |
Actin interaction |
Selective staining of actin filaments in vitro. |
Properties
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|
Form |
Dry |
Quantity |
1x 100 Units (sufficient for 100 slides) |
Buffer |
Reconstitute in 100µl MeOH, DMSO or DMF. Dilute 5µl of the phalloidin stock with 500µl PBS and apply to stain the sample. |
Sub cellular localization |
Actin, Cytoskeleton |
Excitation/Emission |
ex 496 nm, em 661 nm |
Kit Content |
1x 100 Units ActinStain ATTO490LS; |
Storage instructions |
Upon reconstitution the product is stored at -5 to -70°C. Always protect from light. |
Shipping conditions |
At ambient temperature. Upon delivery store at -5 to -70°C. |
Remarks |
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CAS no. |
N/A |
Further Information
References
Septins guide microtubule protrusions induced by actin-depolymerizing toxins like Clostridium difficile transferase (CDT).
Nölke T, Schwan C, Lehmann F, Østevold K, Pertz O, Aktories K.
Proc Natl Acad Sci U S A. 2016 Jul 12;113(28)
Septins guide microtubule protrusions induced by actin-depolymerizing toxins like Clostridium difficile transferase (CDT).
Nölke T, Schwan C, Lehmann F, Østevold K, Pertz O, Aktories K.
Proc Natl Acad Sci U S A. 2016 Jul 12;113(28)
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