ActinStain ATTO 565
Cat. #: 8813-01
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Applications
Rapid staining of single actin filaments with highly photostable 565-Phalloidin.
Replacement for: Alexa Fluor® 555, Cy™3,Tetramethylrhodamine.
Description
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|
Product |
ActinStain ATTO565 |
Origin |
Amanita Phalloides Conjugate |
Molecular mass |
N/A |
Product description |
Rapid and selective staining of single actin filaments with highly photostable ActinStain ATTO565 is based on the selective affinity of phalloidin for filamentous actin. Phalloidin is a bicyclic peptide of a family of toxins isolated from the Amanita phalloides “death cap” mushroom. The peptide is commonly used as a selective stain or counterstain to specifically label F-actin in fixed or permeabilized cells, and cell-free experiments. ActinStain ATTO565 has a strong orange fluorescence of high brightness and superior photostability. |
Actin interaction |
Selective staining of actin filaments in in vitro. |
Description
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|
Form |
Dry |
Quantity |
1x 100 Units |
Buffer |
Reconstitute in 100µl MeOH, DMSO or DMF. Mix 5µl of the Phalloidin stock with 125µl 1x PolyMix. The suitable volume added to the sample has to be determined individually (mix the phalloidin solution approximately 1+1 to 3+1 with the actin filaments). |
Application |
F-Actin solution |
Excitation/Emission |
ex 563 nm, em 592 nm |
Kit Content |
1x 100 Units ActinStain ATTO565 |
Storage instructions |
Upon reconstitution the product is stored at -5 to -70°C. Always protect from light. |
Shipping conditions |
At ambient temperature. Upon delivery store at -5 to -70°C. |
Remarks |
|
CAS no. |
N/A |
Further Information
Septins guide microtubule protrusions induced by actin-depolymerizing toxins like Clostridium difficile transferase (CDT).
Nölke T, Schwan C, Lehmann F, Østevold K, Pertz O, Aktories K.
Proc Natl Acad Sci U S A. 2016 Jul 12;113(28)
Septins guide microtubule protrusions induced by actin-depolymerizing toxins like Clostridium difficile transferase (CDT).
Nölke T, Schwan C, Lehmann F, Østevold K, Pertz O, Aktories K.
Proc Natl Acad Sci U S A. 2016 Jul 12;113(28)
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