Product

ActinStain ATTO565

Origin

Amanita Phalloides Conjugate

Molecular mass

N/A

Product description

Rapid and selective staining of single actin filaments with highly photostable ActinStain ATTO565 is based on the selective affinity of phalloidin for filamentous actin. Phalloidin is a bicyclic peptide of a family of toxins isolated from the Amanita phalloides “death cap” mushroom. The peptide is commonly used as a selective stain or counterstain to specifically label F-actin in fixed or permeabilized cells, and cell-free experiments.

Labeled phalloidin conjugates bind in a stoichiometric ratio of about one phallotoxin per actin subunit in both muscle and non-muscle cells, and have similar affinity for both large and small filaments. Phalloidins do not bind to monomeric actin and have a stabilizing effect on F-actin. 
 

ActinStain ATTO565 has a strong orange fluorescence of high brightness and superior photostability.

Actin interaction

Selective staining of actin filaments in in vitro.

Form

Dry

Quantity

1x 100 Units 

Buffer

Reconstitute in 100µl MeOH, DMSO or DMF. Mix 5µl of the Phalloidin stock with 125µl 1x PolyMix. The suitable volume added to the sample has to be determined individually (mix the phalloidin solution approximately 1+1 to 3+1 with the actin filaments).

Application

F-Actin solution

Excitation/Emission

ex 563 nm, em 592 nm

Kit Content

1x 100 Units  ActinStain ATTO565
1x 1 ml PolyMix (10x conc., F-actin buffer, lyophilized);

Storage instructions

Upon reconstitution the product is stored at -5 to -70°C. Always protect from light.

Shipping conditions 

At ambient temperature. Upon delivery store at -5 to -70°C.

Remarks

CAS no.

 N/A