ActinStain ATTO594
Cat. #: 8815-01
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Applications
Rapid staining of single actin filaments with highly photostable ActinStain ATTO594.
Description
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Product
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ActinStain ATTO594
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Origin
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Amanita Phalloides Conjugate
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Molecular mass
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N/A
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Product description
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Rapid and selective staining of single actin filaments with highly photostable ActinStain ATTO488 is based on the selective affinity of phalloidin for filamentous actin. Phalloidin is a bicyclic peptide of a family of toxins isolated from the Amanita phalloides “death cap” mushroom. The peptide is commonly used as a selective stain or counterstain to specifically label F-actin in fixed or permeabilized cells, and cell-free experiments. Labeled phalloidin conjugates bind in a stoichiometric ratio of about one phallotoxin per actin subunit in both muscle and non-muscle cells, and have similar affinity for both large and small filaments. Phalloidins do not bind to monomeric actin and have a stabilizing effect on F-actin.
ActinStain ATTO594 has a strong orange-red fluorescence of high brightness and superior photostability.
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Actin interaction
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Selective staining of actin filments in vitro.
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Properties
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Form
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Dry
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Quantity
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1x 100 Units
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Buffer
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Reconstitute in 100µl MeOH, DMSO or DMF. Dilute 5µl of the phalloidin stock with 500µl PBS and apply to stain the sample.
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Sub cellular localization
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Actin, Cytoskeleton
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Excitation/Emission
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ex 601 nm, em 627 nm
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Kit Content
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1x100 Units ActinStain ATTO594:
1x PolyMix (10x conc., F-actin buffer, lyophilized);
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Storage instructions
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Upon reconstitution the product is stored at -5 to -70°C. Always protect from light.
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Shipping conditions
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At ambient temperature. Upon delivery store at -5 to -70°C.
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Remarks
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CAS no.
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N/A
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Further Information
Table of Dyes and Light Sources
References
Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms
Gordon David E., et al.
Science. 2020 Dec 4; 370(6521): eabe9403.
Septins guide microtubule protrusions induced by actin-depolymerizing toxins like Clostridium difficile transferase (CDT).
Nölke T, Schwan C, Lehmann F, Østevold K, Pertz O, Aktories K.
Proc Natl Acad Sci U S A. 2016 Jul 12;113(28)