ActinStain ATTO 647
Cat. #: 8817-01
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Applications
Rapid staining of single actin filaments with highly photostable ActinStain ATTO647.
Description
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|
Product |
ActinStain ATTO647 |
Origin |
Amanita Phalloides Conjugate |
Molecular mass |
N/A |
Product description |
Rapid and selective staining of single actin filaments with highly photostable ActinStain ATTO647 is based on the selective affinity of phalloidin for filamentous actin. Phalloidin is a bicyclic peptide of a family of toxins isolated from the Amanita phalloides “death cap” mushroom. The peptide is commonly used as a selective stain or counterstain to specifically label F-actin in fixed or permeabilized cells, and cell-free experiments. ActinStain ATTO647 has a strong orange-red fluorescence of high brightness and superior photostability. |
Actin interaction |
Selective staining of actin filments in vitro. |
Properties
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|
Form |
Dry |
Quantity |
1x 100 Units |
Buffer |
Reconstitute in 100µl MeOH, DMSO or DMF. Dilute 5µl of the phalloidin stock with 500µl PBS and apply to stain the sample. |
Sub cellular localization |
Actin, Cytoskeleton |
Excitation/Emission |
ex 647 nm, em 669 nm |
Kit Content |
1x100 Units ActinStain ATTO647: |
Storage instructions |
Upon reconstitution the product is stored at -5 to -70°C. Always protect from light. |
Shipping conditions |
At ambient temperature. Upon delivery store at -5 to -70°C. |
Remarks |
|
CAS no. |
N/A |
Further Information
Septins guide microtubule protrusions induced by actin-depolymerizing toxins like Clostridium difficile transferase (CDT).
Nölke T, Schwan C, Lehmann F, Østevold K, Pertz O, Aktories K.
Proc Natl Acad Sci U S A. 2016 Jul 12;113(28)
Septins guide microtubule protrusions induced by actin-depolymerizing toxins like Clostridium difficile transferase (CDT).
Nölke T, Schwan C, Lehmann F, Østevold K, Pertz O, Aktories K.
Proc Natl Acad Sci U S A. 2016 Jul 12;113(28)
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