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Actin — an example of controlled quality
Hypermol® actin is manufactured with batch-level quality control to support reproducible experimental performance. The points below summarize the quality controls and manufacturing principles used for skeletal muscle actin.
Controlled source preparation
Skeletal muscle actin prepared from a single defined muscle type (musculus latissimus dorsi), to reduce biological variability.
Purity & concentration control
Purity assessed by scanning densitometry of Coomassie Blue G-250 stained SDS gels (15%). Protein concentration determined routinely by UV-VIS spectroscopy.
Functional polymerization checks
Polymerization performance of lyophilized actin is assessed batchwise using Ostwald viscometry and fluorometry with pyrene actin (10%).
Activity retention after lyophilization
Example benchmark: steady-state viscosity is no less than 95% of the actin prior to freeze-drying (batchwise QC criterion as stated).
Preservation approach
A dedicated cryoconservation method is used to preserve structural integrity and restore biological activity after storage and handling.
