Alpha-Actinin 1 (smooth muscle) - 2x 100 µg
α-Actinin
Native actin-binding protein from smooth muscle
Description
| Protein | α-Actinin |
| Origin | Smooth muscle, turkey gizzard |
| Molecular mass | ~200 kDa (homodimer of 2 × 100 kDa subunits) |
| Protein description | α-Actinin is a native actin cross-linking protein of the spectrin superfamily. In smooth muscle it forms a homodimer (~200 kDa total molecular mass). Four isoforms are known: isoforms 2 and 3 represent the “muscle” α-actinins localized in sarcomeres of skeletal and cardiac muscle, whereas isoforms 1 and 4 are “non-muscle” variants broadly expressed in multiple tissues. |
| Actin interaction | Actin cross-linking protein; Kd (F-actin binding) = 1–5 µM. |
Properties
| Form | Lyophilized protein, ready-to-use after reconstitution. |
| Quantity per unit | 1.0 mg |
| Buffer (after reconstitution) | 50 mM NaCl, 20 mM Tris-HCl pH 7.4, 0.5 mM DTT, 3% disaccharides (1 mg/ml after addition of 1 ml ultrapure water). |
| Purity | >97% by scanning densitometry of Coomassie G-250 stained SDS-PAGE. |
| Purification notes | Native protein purified from turkey gizzard smooth muscle by ion-exchange chromatography (IEX) and size-exclusion chromatography (SEC). |
| Protein concentration | Determined by UV/VIS spectroscopy at A280 (A280 = 0.1201 for 1 mg/ml). |
| Storage instructions | After reconstitution store on ice for short term. For long-term storage prepare glycerol stocks and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. Upon receipt store at -70°C. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
| CAS no. | |
Proteins and buffers from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product Data Sheet
Material and Safety Data Sheet
Protein Sequence on NCBI (chain A)
Protein Sequence on NCBI (chain B)
Selected References
Reconstitution of contractile actomyosin rings in vesicles.
Nat Commun. 2021;12:2254.
Real-time dynamics of emerging actin networks in cell-mimicking compartments.
PLoS One. 2015;10:e0116521.
A high molecular mass protein isolated from chicken gizzard: its localization at the dense plaques and dense bodies of smooth muscle and the Z-disks of skeletal muscle.
J Cell Sci. 1995;108:857–868.
