Alkaline Phosphatase Buffer (10x)
pH 9.5
Cat. #: 6022-01
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Description
Alkaline Phosphatase Buffer (10x) is formulated for use with alkaline phosphatase-based detection systems in Western blot applications.
It is suitable for both nitrocellulose and PVDF membranes and is optimized for chromogenic and chemiluminescent detection methods.
This buffer is designed for use during the final washing step prior to substrate addition and supports high-sensitivity detection in a wide range
of experimental protocols. It is compatible with substrates such as BCIP/NBT and CSPD, ensuring flexibility in experimental design.
Product Details
- Concentration: 10x
- Volume: 100 ml
- Application: Western blotting with alkaline phosphatase detection systems
- Membrane Compatibility: Nitrocellulose and PVDF
- Coverage: Provides sufficient volume to develop approximately 500 cm² of blotting membrane
This buffer serves as a practical alternative to Enhanced Chemiluminescence (ECL) systems, offering reliable
and reproducible performance across diverse laboratory workflows.
Content
100ml Alkaline Phosphatase Buffer (10x concentrate)
Stability & Storage
Stable for a minimum of 2 years, when stored at 20°C.
