AMP-PNP | 100mM, pH 7.4

Product name

AMP-PNP, Synonyms: Adenosine-5'-[(β,γ)-imido]triphosphate, Tetralithium salt

Product description

Adenosine-5'-[(β,γ)-imido]triphosphate (AMP-PNP) is a non-hydrlyzale analogue of ATP, used to study ATPase activities, energy storage and metabolism in vitro and to keep ATPases constitutively active. Proteins like actin and myosin have a functional ATPase activity, where AMP-PNP is used for analysis.

Overview

Suited to supplement all buffers and solutions buffered at ~pH 7.4.

Form

Lyophilized, ready-to-use.

Quantity

Powder: 100mM AMP-PNP, 50mM Hepes titrated with NaOH to pH 7.4. Sterile filtered before freeze-drying. Weigh into the tube 50 mg of ultrapure H₂O, corresponding to 50 µl ultrapure H₂O, to obtain a fresh 100mM stock solution.

Formula

Empirical Formula (Hill Notation) C₁₀H₁₇N₆O₁₂P₃(free acid); Molecular Weight 506.19 g/mol of the free acid without buffer.

Purity

AMP-PNP  ≥ 99 % (HPLC). Ultrapure buffer reagents.

Storage instructions

AMP-PNP is stored at –20°C upon arrival. The product will be stable in performance for at least 1 year from the date of purchase. Solubilized AMP-PNP can be aliquoted and stored at -70°C for use within 3 months. Thawed aliquots should be used within 1 week. Avoid refreezing.   

Shipping conditions 

At ambient temperature. Upon delivery store at -20°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

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