Monoclonal Anti-Actin (clone 2G2)

Product name

Monoclonal anti-actin (clone 2G2)

Target species

skeletal and nuclear non-muscle actin: PtK2, SR-NRK, NRK-49F, L6 cells, C2C12, NIH-3T3, rabbit myoblast, myotubes

Description

Monoclonal anti actin (98% purity) recognizes skeletal and nuclear actin (non-muscle actin isoforms). Although isotype-classified as IgM, it reacts even to stronger with goat-anti mouse IgG. In immunofluorescence microscopy samples are fixed with methanol to detect cytoplasmic actin, while fixation with para-formaldehyde leads to nuclear actin detection (Gonsior et al.,, 1999).

As immunogen for mono-anti actin a profiling-actin complex from calf thymus was used, and epitope mapping localized the following sequence (Gonsior et al., 1999):

NVPAMYVAVLDSGVTHNVPIYHAIMRLDLA.

Mono-anti actin was tested on PtK2, SR-NRK, NRK-49F, L6 cells, C2C12, NIH-3T3, rabbit myoblast and myotube cells.

Specification

Immunofluorescence: 1:100-1:500, 
Immunoblot: 1:500-1500.

Optical Properties

Form

Freeze-dried.

Quantity

250µg

Content


Purity

Buffer recommendation

250µg monoclonal Anti-Actin (clone 2G2)
Product documentation

99% pure (IEX-chromatography)

50% glycerol, 3% BSA, 0.01 M sodium phosphate, 0.1 M 
sodium chloride, 5 mM sodium azide, pH 7.4.

Clonality

Monoclonal

Isotype

IgM (reacts as IgM but also IgG, a novel feature of some IgMs)

Storage instructions

Store as glyecrol stock at -20°C, Avoid freeze/thaw cycles.

Remarks

CAS no.

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Processes.