
Monoclonal Anti-Actin
clone 2G2, >95% purity
Cat. # 7301-01
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Description
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Product name
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Monoclonal anti-actin (clone 2G2)
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Target species
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skeletal and nuclear non-muscle actin: PtK2, SR-NRK, NRK-49F, L6 cells, C2C12, NIH-3T3, rabbit myoblast, myotubes
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Description
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Monoclonal anti actin (98% purity) recognizes skeletal and nuclear actin (non-muscle actin isoforms). Although isotype-classified as IgM, it reacts even to stronger with goat-anti mouse IgG. In immunofluorescence microscopy samples are fixed with methanol to detect cytoplasmic actin, while fixation with para-formaldehyde leads to nuclear actin detection (Gonsior et al.,, 1999).
As immunogen for mono-anti actin a profiling-actin complex from calf thymus was used, and epitope mapping localized the following sequence (Gonsior et al., 1999):
NVPAMYVAVLDSGVTHNVPIYHAIMRLDLA.
Mono-anti actin was tested on PtK2, SR-NRK, NRK-49F, L6 cells, C2C12, NIH-3T3, rabbit myoblast and myotube cells.
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Specification
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Immunofluorescence: 1:100-1:500,
Immunoblot: 1:500-1500.
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Optical Properties
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Properties
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Form
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Freeze-dried.
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Quantity
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250µg
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Content
Purity
Buffer recommendation
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250µg monoclonal Anti-Actin (clone 2G2)
Product documentation
99% pure (IEX-chromatography)
50% glycerol, 3% BSA, 0.01 M sodium phosphate, 0.1 M
sodium chloride, 5 mM sodium azide, pH 7.4.
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Clonality
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Monoclonal
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Isotype
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IgM (reacts as IgM but also IgG, a novel feature of some IgMs)
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Storage instructions
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Store as glyecrol stock at -20°C, Avoid freeze/thaw cycles.
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Remarks
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CAS no.
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Processes.
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References
Septin remodeling is essential for the formation of cell membrane protrusions (microtentacles) in detached tumor cells.
Østevold K, Meléndez AV, Lehmann F, Schmidt G, Aktories K, Schwan C.
Oncotarget. 2017 Sep 11;8(44):76686-76698. doi: 10.18632/oncotarget.20805. eCollection 2017 Sep 29.
Initiation of DNA replication requires actin dynamics and formin activity.
Parisis N, Krasinska L, Harker B, Urbach S, Rossignol M, Camasses A, Dewar J, Morin N, Fisher D.
EMBO J. 2017 Nov 2;36(21):3212-3231. doi: 10.15252/embj.201796585. Epub 2017 Oct 5.