Monoclonal Anti-Actin (clone 2G2)
Monoclonal Anti-Actin
clone 2G2, mouse monoclonal primary antibody
Primary antibody Actin clone 2G2 IF and immunoblot Research use only
Description
| Product name | Monoclonal Anti-Actin (clone 2G2) |
| Product type | Mouse monoclonal primary antibody against actin |
| Target / specificity | Clone 2G2 recognizes skeletal and non-muscle actin isoforms and has been used to detect cytoplasmic as well as nuclear actin forms. The antibody is conformation-sensitive and recognizes an epitope region that is accessible in profilin-complexed G-actin, but not exposed on the surface of filamentous F-actin. |
| Tested cells | Tested on PtK2, SR-NRK, NRK-49F, L6, C2C12, NIH-3T3, rabbit myoblasts and myotubes. |
| Immunogen | Profilin-actin complex from calf thymus. |
| Fixation notes | In immunofluorescence microscopy, methanol fixation supports detection of cytoplasmic actin, whereas paraformaldehyde fixation has been reported to reveal nuclear actin staining with clone 2G2. |
| Applications | Immunofluorescence microscopy; immunoblotting. |
| Recommended dilution | Immunofluorescence: 1:100–1:500 Immunoblot: 1:500–1:1500 |
| Molecular target | Actin, approximately 42–43 kDa |
Clone 2G2 is particularly useful when actin conformation and fixation-dependent accessibility are relevant to the experimental question. Although classified as IgM, clone 2G2 is known to be very well detectable with secondary anti-mouse IgG antibodies and is also detected by anti-mouse IgM secondaries. This can be convenient if no dedicated anti-mouse IgM secondary antibody is available. As with all primary antibodies, optimal dilution and fixation conditions should be validated for the specific cell type and assay setup.
Properties
| Form | Freeze-dried. |
| Quantity per unit | 250 µg |
| Content | 250 µg monoclonal Anti-Actin (clone 2G2) |
| Purity | >95%; purified by ion-exchange chromatography. |
| Clonality | Monoclonal |
| Host species | Mouse |
| Isotype and secondary antibody detection | IgM. Clone 2G2 is known to be very well detectable with secondary anti-mouse IgG antibodies, although it is classified as an IgM antibody. It is also detected by secondary anti-mouse IgM antibodies. This is a practical advantage if anti-mouse IgM secondaries are not available in the laboratory, since additional anti-IgM secondary antibodies are usually not required for detection. |
| Buffer recommendation | 50% glycerol, 3% BSA, 0.01 M sodium phosphate, 0.1 M sodium chloride, 5 mM sodium azide, pH 7.4. |
| Storage instructions | Store as glycerol stock at –20°C. Avoid repeated freeze-thaw cycles. |
| Shipping conditions | According to current Hypermol® shipping conditions for primary antibodies. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic, therapeutic, clinical or in vivo applications. |
Hypermol® primary antibodies are provided for research use only applications and should be handled according to the corresponding product documentation and safety data sheet.
Further Information
Product DataSheet Monoclonal Anti-Actin, clone 2G2
Material and Safety Data Sheet Safety information for handling and storage
References
