Anti-HA ATTO647 (clone 12CA5)

Product name

Optical properties

Anti-HA ATTO647  (clone 12CA5)

λ_ex 647nm, λ_em 669nm 

Target 

Immunogen :  synthetic peptide corresponding to hemagglutinin aa 98-106 of human influenca virus.
Sequence : Epitope sequence, YPYDVPDYA .

Description

The HA Tag (hemagglutinin tag) is an epitope tag, commonly used to localize gene products in many types of cultured cells, to study the topology of proteins and protein complexes. Anti-HA is especially useful whenever no primary antibody against the protein of interest is available, mostly because it is less immunogenic and low abundant.
 
Monoclonal Anti-HA is directed against a peptide epitope of the hemagglutinin (HA) glycoprotein derived from recombinant hemagglutinin protein (aa 98-106) of the human influenza virus. The antibody is used for the immunodetection and functional characterization of native and denatured protein. It is frequently used for the detection and characterization of recombinant expressed HA-tagged proteins in vivo and in vitro or proteins containing the epitope YPYDVPDYA. Anti-HA facilitates protein detection in all blotting techniques, immunofluorescence & -chemistry and co-immunoprecipitation of tagged proteins

Specification

Immunofluorescence (IF) . 5-10 µg/mL
Immunohistochemistry (Paraffin) (IHC (P)) : 1:100
Immunoprecipitation (IP) : 4-8 µg
ELISA (ELISA) .: 0.2-2.0 µg/mL
Western Blot (WB) : 1 µg/mL

Synonyms

Form

Freeze-dried

Quantity

200 µg

Product content
 

200 µg Anti-HA ATTO647 (clone 12CA5)
1 ml Glycerol-PBS (Reconstitution Buffer)

Clonality

Monoclonal

Isotype

Mouse IgG2b

Storage instructions

Store as glyecrol stock at -20°C, Avoid freeze/thaw cycles.

Remarks

CAS no.

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Processes.