Seven subunit actin branching protein complex
Cat. #: 8413-01
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Description
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|
Protein |
Arp 2/3 protein complex |
Origin |
brain, porcine |
Molecular mass |
224 kDa |
Protein description |
The seven-subunit protein complex comprises Arp2 and Arp3 and five smaller (Arc) proteins. Both Arp2 and Arp3 sequences possess nucleotide-binding and divalent cation-binding sites. Arp 2/3 complex consists of five associated proteins to Arp2 and Arp3, namely p16, p20, p21, p34, and p41 (Synonyms: ARPC-5, -4, -3, -2, and -1).
Arp2/3 is a constitutively inactive complex. The actin nucleation is efficiently activated in vitro by binding of the VCA-domains (Cat. #: 8416-01) of members of the Wiskott-Aldrich syndrome family protein, like WAVE (WASP family verprolin homologous). |
Actin interaction |
Pointed end capping protein. Branching of actin filaments. Strong Nucleation upon activation by VCA-domain (Cat. #: 8416-01) |
Properties
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|
Form |
Lyophilized, ready-to-use. |
Quantity per unit |
2x 50 µg |
Buffer |
150mM KCl, 20mM Trizma pH 7.5, 1mM DTT, 0.2mM ATP, 0.5mM EDTA, 1mM MgCl2 and 5% disaccharides, when reconstituted with 50µl ultrapure water to obtain a 1 mg/ml solution. |
Purity |
>98% by scanning densitometry from Coomassie G-250 stained SDS-Gels. |
Purification notes |
Purified from porcine brain. |
Protein concentration |
Determined at OD280 (0.1%=1.033). |
Storage instructions |
Arp2/3 complex is stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized Arp2/3 complex is kept on ice and should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01). Avoid repeated freeze / thaw cycles. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
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Further Information
Product DataSheet
Material and Safety Data Sheet
Protein Sequence on NCBI (All chain sequences)
References
Initiation of DNA replication requires actin dynamics and formin activity.
Parisis N, Krasinska L, Harker B, Urbach S, Rossignol M, Camasses A, Dewar J, Morin N, Fisher D.
EMBO J. 2017 Nov 2;36(21):3212-3231. doi: 10.15252/embj.201796585. Epub 2017 Oct 5.
Polarized Exocytosis Induces Compensatory Endocytosis by Sec4p-Regulated Cortical Actin Polymerization.
Johansen J, Alfaro G, Beh CT.
PLoS Biol. 2016 Aug 15;14(8):e1002534. doi: 10.1371/journal.pbio.1002534. eCollection 2016 Aug.
Crystal structures of actin-related protein 2/3 complex with bound ATP or ADP.
Nolen BJ, Littlefield RS, Pollard TD.
Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15627-32. Epub 2004 Oct 25.
Reconstitution of contractile actomyosin rings in vesicles.
Litschel T, Kelley CF, Holz D, Adeli Koudehi M, Vogel SK, Burbaum L, Mizuno N, Vavylonis D, Schwille P.Nat Commun. 2021 Apr 15;12(1):2254. doi: 10.1038/s41467-021-22422-7.
Initiation of DNA replication requires actin dynamics and formin activity.
Parisis N, Krasinska L, Harker B, Urbach S, Rossignol M, Camasses A, Dewar J, Morin N, Fisher D.
EMBO J. 2017 Nov 2;36(21):3212-3231. doi: 10.15252/embj.201796585. Epub 2017 Oct 5.
Polarized Exocytosis Induces Compensatory Endocytosis by Sec4p-Regulated Cortical Actin Polymerization.
Johansen J, Alfaro G, Beh CT.
PLoS Biol. 2016 Aug 15;14(8):e1002534. doi: 10.1371/journal.pbio.1002534. eCollection 2016 Aug.
Crystal structures of actin-related protein 2/3 complex with bound ATP or ADP.
Nolen BJ, Littlefield RS, Pollard TD.
Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15627-32. Epub 2004 Oct 25.
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