Protein

Arp 2/3 protein complex

Origin

brain, porcine

Molecular mass

224 kDa

Protein description

The seven-subunit protein complex comprises Arp2 and Arp3 and five smaller (Arc) proteins. Both Arp2 and Arp3 sequences possess nucleotide-binding and divalent cation-binding sites. Arp 2/3 complex consists of five associated proteins to Arp2 and Arp3, namely p16, p20, p21, p34, and p41 (Synonyms: ARPC-5, -4, -3, -2, and -1). 

Arp2/3 is a constitutively inactive complex. The actin nucleation is efficiently activated in vitro by binding of the VCA-domains (Cat. #: 8416-01) of members of the Wiskott-Aldrich syndrome family protein, like WAVE (WASP family verprolin homologous). 

Actin interaction

Pointed end capping protein. Branching of actin filaments. Strong Nucleation upon activation by VCA-domain (Cat. #: 8416-01)  

Form

Lyophilized, ready-to-use.

Quantity per unit

2x 50 µg

Buffer

150mM KCl, 20mM Trizma pH 7.5, 1mM DTT, 0.2mM ATP, 0.5mM EDTA, 1mM MgCl2 and 5% disaccharides, when reconstituted with 50µl ultrapure water to obtain a 1 mg/ml solution.

Purity

>98% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Purification notes

Purified from porcine brain.

Protein concentration

Determined at OD280 (0.1%=1.033).

Storage instructions

Arp2/3 complex is stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized Arp2/3 complex is kept on ice and should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01).  Avoid repeated freeze / thaw cycles. 

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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