ATPγS | 100mM, pH 7.4
ATPγS
100 mM, pH 7.4
Description
| Product name | ATPγS, Synonyms: Adenosine-5'-(3-thio)-triphosphate (tetralithium salt), Adenosine-5'-(3-thiotriphosphate) |
| Product description | Adenosine-5'-(3-thio)-triphosphate (ATPγS) is used as a non-hydrolyzable analogue of ATP to study ATP-dependent processes in vitro. Proteins such as actin and myosin have functional ATPase activity; ATPγS is used to maintain ATP-binding states without rapid turnover by ATP hydrolysis. |
| Overview | Suited to supplement all buffers and solutions buffered at ~pH 7.4. |
Properties
| Form | Lyophilized, ready-to-use. |
| Quantity | Powder: 100 mM ATPγS, 50 mM Hepes titrated with NaOH to pH 7.4. Sterile filtered before freeze-drying. Weigh into the tube 50 mg of ultrapure H2O, corresponding to 50 µl ultrapure H2O, to obtain a fresh 100 mM stock solution. |
| Formula | Empirical Formula (Hill Notation) C10H16N5O14P3S (free acid); Molecular Weight 539.23 of the pure compound without buffer. |
| Purity | ATPγS ≥ 95% (HPLC); 5% ADP. Ultrapure buffer reagents. |
| Storage instructions | ATPγS is stored at −20 °C upon arrival. The product will be stable in performance for at least 1 year from the date of purchase. Solubilized ATPγS can be aliquoted and stored at −70 °C for use within 3 months. Thawed aliquots should be used within 1 week. Avoid refreezing. |
| Shipping conditions | At ambient temperature. Upon delivery store at −20 °C. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
| CAS no. | |
Proteins and buffers from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
