Biotin-Actin
Cat. #: 8109-01
α-actin from skeletal muscle, rabbit
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Description
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Protein |
Biotin-Actin |
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Origin |
Skeletal muscle alpha actin, rabbit |
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Molecular mass |
42kDa |
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Protein description |
Biotin-Actin from rabbit skeletal muscle is chemically modified α-actin by esterification of lysine residues with biotin. The advantage of Biotin-Actin from Hypermol is, that it is equipped with a ~20 Å spacer, which renders the Biotin group to be readily available for antibody detection in all suitable techniques (ELISA, Western Blotting etc.). |
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Conjugation ratio |
The degree of labelling (DOL) of Biotin-Actin is approx. 1 . |
Properties
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Form |
Lyophilized, ready-to-use. |
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Quantity |
2x100 µg |
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Buffer |
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100 µl ultrapure water to obtain a 1mg/ml solution. |
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Purity |
>99% by scanning densitometry. |
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Purification notes |
Purified by GPC from rabbit skeletal muscle. |
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Protein concentration |
Determined by the Biuret method. |
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Storage instructions |
Biotin-Actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be used within 1 week. Avoid refreezing. |
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Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
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Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
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CAS no. |
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Further Information
Product DataSheet
Material and Safety Data Sheet
Protein Sequence on NCBI
References
Phosphoinositides regulate force-independent interactions between talin, vinculin, and actin.
Kelley CF, Litschel T, Schumacher S, Dedden D, Schwille P, Mizuno N.; Elife. 2020 Jul 13;9:e56110. doi: 10.7554/eLife.56110. PMID: 32657269; PMCID: PMC7384861.
Reconstitution of contractile actomyosin rings in vesicles.
Litschel, T., Kelley, C.F., Holz, D. et al.; Nat Commun 12, 2254 (2021). https://doi.org/10.1038/s41467-021-22422-7
Product DataSheetMaterial and Safety Data Sheet
Protein Sequence on NCBI
Phosphoinositides regulate force-independent interactions between talin, vinculin, and actin.
Kelley CF, Litschel T, Schumacher S, Dedden D, Schwille P, Mizuno N.; Elife. 2020 Jul 13;9:e56110. doi: 10.7554/eLife.56110. PMID: 32657269; PMCID: PMC7384861.
Reconstitution of contractile actomyosin rings in vesicles.
Litschel, T., Kelley, C.F., Holz, D. et al.; Nat Commun 12, 2254 (2021). https://doi.org/10.1038/s41467-021-22422-7
