Biuret Reagent
Biuret
Biuret reagent; ready-to-use concentrate.
Description
| Product name | Biuret reagent |
| Assay principle | The Biuret assay determines protein concentration by complexation of peptide bonds with copper(II) ions under alkaline conditions. The protein concentration is quantified by colorimetric measurement at 540–550 nm. |
| Product description | Biuret reagent is used for the determination of soluble and insoluble proteins. The alkaline assay environment is provided by NaOH in the protein solution, allowing protein determination under conditions where acidic assays, such as Bradford-type assays, may be less suitable for insoluble proteins. The method is commonly used when larger sample volumes are available and moderate assay sensitivity is sufficient. The working range is approximately 0.1–5 mg/ml protein. |
| Detection | Colorimetric measurement at 540–550 nm. |
| Sensitivity | >0.1 mg/ml protein. |
Biuret complex
Properties
| Form | Ready-to-use concentrate. |
| Content | 50 ml Biuret solution, standardized solution. |
| Assay capacity | Sufficient for approximately 160 assays in half-micro cuvettes using 630 µl assay volume, or more than 800 assays using micro cuvettes or microtiter plates. |
| Microplate protocol | Mix 60 µl protein solution with 66 µl Biuret solution. Incubate for 20–30 min and measure at 540–550 nm using a microplate reader. |
| Half-micro cuvette protocol | Mix 300 µl protein solution with 330 µl Biuret solution. Incubate for 20–30 min and measure at 540–550 nm using micro cuvettes. |
| Reference | Sample buffer or H2O. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
