Protein

Caldesmon (h-Caldesmon)

Origin

smooth muscle, turkey

Molecular mass

~87 kDa

Protein description

Caldesmon from gizzard smooth muscle is the high molecular weight isoform (793 aa, 87-89 kDa, h-CaD), constituting a component of the thin filaments of the contractile apparatus. Caldesmon is a multifunctional protein with binding sites for actin, myosin, tropomyosin and calcium binding proteins, e.g. calmodulin. It is phosphorylated by a number of kinases. In smooth muscle caldesmon is a modulator of the actin filament activation of the myosin ATPase, motility and contractility.

Actin interaction

Binds to and bundles actin filaments; Caldesmon & tropomyosin inhibit binding of fascin.

Form

Lyophilized, ready-to-use.

Quantity per unit

2x50µg

Buffer

100mM KCl, 10mM imidazole pH 7.4, 2mM MgCl₂, 0.2mM DTT and 3% disaccharides, when reconstituted in 50µl ultrapure water.

Purity & Activity

Purity: >98% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Purification notes

Caldesmon was purified from turkey gizzard and finally purified by gel filtration chromatography on  Superdex 200 . 

Protein concentration

Determined by OD280 (0.1%=1.224).

Storage instructions

Caldesmon is stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01). Avoid repeated freeze / thaw cycles. 

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

CAS no.