CapZ (non-muscle, human recombinant) - 50 µg

Protein

Cap Z, Capping protein (non-muscle isoform)

Origin

recombinant, human

Molecular mass

66kDa

Protein description

Non-Muscle CapZ Heterodimer is a high-affinity actin filament capping protein essential for cytoskeletal organization and cellular dynamics. This heterodimeric protein consists of two subunits:

• α1-subunit (34 kDa)

• β2-subunit (32 kDa)

CapZ binds with nanomolar affinity to the barbed ends of actin filaments in a Ca²⁺-independent manner, effectively regulating actin filament growth and turnover. By capping the fast-growing barbed end, CapZ prevents the addition or dissociation of actin monomers, ensuring precise filament length control and stabilization.

Actin interaction

Ca⁺⁺-insensitive barbed end capping protein. 

Form

Lyophilized, ready-to-use.

Quantity per unit

50 µg

Buffer

150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 50 µl ultrapure water to obtain a 1.0 mg/ml solution.

Purity & Activity

Purity: >94% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Capping activity: F-actin (Cat.#:8101) containing 20% pyrene actin (Cat.#: 8112) was incubated with CapZ and diluted below the critical concentration. Depolymerisation was followed by fluorometry and compared to an actin reference without CapZ.

Capping was obtained at a molar ratio of 1 : 100 (CapZ : Actin). All proteins were used as supplied, i.e. without dialysis. G-Actin stock solutions were pre-spun 1h, 100.000xg, 4°C.  

Purification notes

96% pure acc. to SDS-PAGE

Protein concentration

Determined by OD280 (0.1%=1.234).

Storage instructions

CapZ is stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20°C in glycerol orCryoProtect (Cat.#: 6011-01). Avoid repeated freeze / thaw cycles. 

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.