ATTO647-Actin (alpha-cardiac actin, bovine) - 5x100µg
ATTO647-Actin
Fluorescent alpha-cardiac actin from bovine cardiac muscle; lysine-labelled with ATTO647.
Applications
| Applications | Fluorescent, polymerization-competent alpha-cardiac actin for fluorescence microscopy, FRET, fluorescence spectroscopy, filament tracking and biochemical actin assays. |
Description
| Product name | ATTO647-Actin |
| Protein | Alpha-cardiac actin, bovine. |
| Molecular mass | 42 kDa. |
| Product description | ATTO647-Actin is chemically modified bovine cardiac G-actin carrying covalently attached ATTO647 fluorescent groups on lysine residues. The protein is prepared from alpha-cardiac actin isolated from bovine cardiac muscle. Cardiac actin is a single-chain polypeptide of approximately 42 kDa consisting of 375 amino acids. ATTO647-Actin is designed for fluorescence-based actin assays and provides a defined fluorescent signal while retaining the polymerization properties required for actin-based biochemical workflows. |
| Fluorophore | ATTO647. |
| Spectral properties | Excitation maximum: 647 nm; emission maximum: 667 nm. |
| Labelling chemistry | ATTO647 is covalently attached to lysine residues of cardiac G-actin. |
| Degree of labelling | This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8. |
| Overview | Fluorescent bovine cardiac G-actin; ATTO647-labelled on lysine residues; polymerization-competent; suitable for fluorescence microscopy, FRET, fluorescence spectroscopy and biochemical actin assays; supplied lyophilized in actin-compatible buffer; 5 × 100 µg package variant. |
Properties
| Form | Lyophilized, ready-to-use after reconstitution. |
| Quantity per unit | 5 × 100 µg. |
| Reconstitution | Reconstitute each 100 µg tube with 100 µl ultrapure water to obtain a 1 mg/ml ATTO647-Actin solution. |
| Buffer | 2 mM Tris-HCl pH 8.2, 0.4 mM ATP, 0.5 mM DTT, 0.1 mM CaCl2, 1 mM NaN3 and 0.3% disaccharides after reconstitution with ultrapure water to obtain a 1 mg/ml solution. |
| Purity | >99% by scanning densitometry. |
| Purification notes | Purified by GPC from bovine cardiac muscle. |
| Protein concentration | Determined by UV absorbance at 290 nm (A290 = 0.63 for 1 mg/ml actin) with fluorophore-specific correction factor applied. |
| Storage instructions | ATTO-Actins stored at −70 °C upon arrival will be stable in performance for at least 6 months from the date of purchase. Solubilized ATTO-Actin should be kept on ice and used within 1 week. Avoid refreezing. |
| Shipping conditions | At ambient temperature. Upon delivery store at −70 °C. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
| CAS no. | |
Proteins and buffers from Hypermol® are prepared with ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Download product datasheet for ATTO647-Actin, 5 × 100 µg pack size.
Material and Safety Data Sheet
MSDS / SDS document for cardiac actin products.
Protein Sequence on NCBI
Reference sequence information for bovine cardiac alpha-actin.
Hide ATTO dyes & spectral properties
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ATTO dyes – spectral overview (λex / λem)
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