Donkey Anti-Goat IgG (H+L) ATTO430LS
Antibody produced in donkey, affinity purified
Cat. #: 2902-1MG
Applications
Suited for fluorescence microscopy and multiplex fluorescence applications. The large Stokes shift supports clear spectral separation, while the preservative-free formulation minimizes interference in sensitive assay conditions.
Description
| Product name | Donkey Anti-Goat IgG (H+L) ATTO430LS | DXG ATTO430LS |
| Target species | Goat |
| Description |
ATTO-labeled secondary antibodies combine high fluorescence intensity, strong photostability and reliable performance in fluorescence-based immunodetection. Donkey Anti-Goat IgG (H+L) ATTO430LS specifically detects goat primary antibodies and is suitable for widefield and confocal fluorescence microscopy. It can also be used in TIRF microscopy when the instrument provides an appropriate excitation source and filter configuration for ATTO430LS. ATTO430LS is a hydrophilic, water-soluble large-Stokes-shift fluorophore with an absorption maximum at 436 nm and a fluorescence maximum at 545 nm. Its fluorescence is efficiently excited within the approximately 400–460 nm spectral range. The exceptionally large Stokes shift of 109 nm results in an almost complete spectral separation of absorption and emission, thereby reducing excitation-light bleed-through into the detection channel. This property is particularly advantageous for multiplex fluorescence imaging and other applications requiring clearly separated excitation and emission windows. The fluorescence quantum yield of approximately 65% is only moderately affected by conjugation to biomolecules. Donkey Anti-Goat IgG (H+L) ATTO430LS is antigen-specific; affinity purification removes essentially all donkey serum proteins, including immunoglobulins not specifically binding to goat IgG. The antibody is conjugated to ATTO430LS and subsequently purified by gel filtration to remove unbound dye, supporting low-background and reproducible fluorescence staining. The product is supplied without preservatives. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2–5, Unbound dye ≤5% of total fluorescence. |
| Optical properties | λex: 436 nm, λem: 545 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is supplied as 1.0 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for reconstitution to 2 mg/ml. After reconstitution, the glycerol stock may be stored at -20°C without freezing. Avoid repeated freeze/thaw cycles. |
| Content | 1.0 mg Donkey Anti-Goat IgG (H+L) ATTO430LS 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification removed donkey serum proteins and immunoglobulins not specifically binding to goat IgG. Following conjugation to ATTO430LS, the antibody was further purified by gel filtration to remove unconjugated dye and low-molecular-weight reaction components. Hypermol® secondary antibodies are highly specific and purified to remove unbound dye, minimizing background signal. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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