Donkey Anti-Goat IgG (H+L) ATTO488
Antibody produced in donkey, affinity purified
Cat. #: 2903-1MG
Applications
Suited for high-sensitivity fluorescence microscopy, flow cytometry and other fluorescence-based applications. The preservative-free formulation minimizes interference in sensitive assay conditions.
Description
| Product name | Donkey Anti-Goat IgG (H+L) ATTO488 | DXG ATTO488 |
| Target species | Goat |
| Description |
ATTO-labeled secondary antibodies combine high fluorescence intensity with strong photostability and reliable performance in fluorescence-based immunodetection. Donkey Anti-Goat IgG (H+L) ATTO488 specifically detects goat primary antibodies and is suitable for widefield, confocal and total internal reflection fluorescence microscopy (TIRF), flow cytometry and related fluorescence-based applications. ATTO488 has an absorption maximum at 501 nm and a fluorescence maximum at 523 nm, producing green fluorescence. The conjugate is efficiently excited within the approximately 480–515 nm range and is well matched to the commonly available 488 nm laser line used in fluorescence microscopes and flow cytometers. Its high fluorescence intensity and strong resistance to photobleaching support sensitive detection, repeated image acquisition and extended fluorescence measurements. ATTO488 is also established for advanced fluorescence-imaging techniques, including structured illumination microscopy (SIM), stimulated emission depletion microscopy (STED), photoactivated localization microscopy (PALM) and direct stochastic optical reconstruction microscopy (dSTORM). The performance of the antibody conjugate in these methods depends on the labeling density, sample preparation, imaging buffer and instrument configuration used for the respective technique. Donkey Anti-Goat IgG (H+L) ATTO488 is antigen-specific; affinity purification removes essentially all donkey serum proteins, including immunoglobulins not specifically binding to goat IgG. Following conjugation to ATTO488, the antibody is further purified by gel filtration to remove unbound dye and support low-background, reproducible fluorescence staining. The product is supplied without preservatives. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2–5, Unbound dye ≤5% of total fluorescence. |
| Optical properties | λabs: 501nm, λfl: 523 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is supplied as 1.0 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for reconstitution to 2 mg/ml. After reconstitution, the glycerol stock may be stored at -20°C without freezing. Avoid repeated freeze/thaw cycles. |
| Content | 1.0 mg Donkey Anti-Goat IgG (H+L) ATTO488 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification removed donkey serum proteins and immunoglobulins not specifically binding to goat IgG. Following conjugation to ATTO488, the antibody was further purified by gel filtration to remove unconjugated dye and low-molecular-weight reaction components. Hypermol® secondary antibodies are highly specific and purified to remove unbound dye, minimizing background signal. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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