Donkey Anti-Goat IgG (H+L) ATTO490LS
Antibody produced in donkey, affinity purified
Cat. #: 2904-1MG
Applications
Suited for multiplex fluorescence microscopy, flow cytometry and related fluorescence-based applications. The large Stokes shift facilitates spectral separation, while the preservative-free formulation minimizes interference in sensitive assay conditions.
Description
| Product name | Donkey Anti-Goat IgG (H+L) ATTO490LS | DXG ATTO490LS |
| Target species | Goat |
| Description |
ATTO-labeled secondary antibodies combine high fluorescence intensity with strong photostability and reliable performance in fluorescence-based immunodetection. Donkey Anti-Goat IgG (H+L) ATTO490LS specifically detects goat primary antibodies and is suitable for widefield and confocal fluorescence microscopy as well as other fluorescence-based immunodetection applications. Its large Stokes shift makes the conjugate particularly valuable for multiplex imaging and applications requiring pronounced spectral separation between excitation and detection channels. ATTO490LS has an absorption maximum at 496 nm and a fluorescence maximum at 661 nm, corresponding to an exceptionally large Stokes shift of 165 nm. The conjugate can be efficiently excited using the widely available 488 nm laser line while its fluorescence is detected in the far-red spectral region. The near-complete separation of the absorption and emission spectra reduces excitation-light bleed-through and enables spectral combinations that are difficult to achieve with conventional fluorophores exhibiting smaller Stokes shifts. ATTO490LS is particularly suitable for multiplex combinations with green-emitting fluorophores such as ATTO488 or ATTO514, because these fluorophores can be excited within a similar spectral range while remaining clearly separated at the emission level. Donkey Anti-Goat IgG (H+L) ATTO490LS is antigen-specific; affinity purification removes essentially all donkey serum proteins, including immunoglobulins not specifically binding to goat IgG. Following conjugation to ATTO490LS, the antibody is further purified by gel filtration to remove unbound dye and support low-background, reproducible fluorescence staining. The product is supplied without preservatives. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2–5, Unbound dye ≤5% of total fluorescence. |
| Optical properties | λabs: 495 nm, λfl: 658 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is supplied as 1.0 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for reconstitution to 2 mg/ml. After reconstitution, the glycerol stock may be stored at -20°C without freezing. Avoid repeated freeze/thaw cycles. |
| Content | 1.0 mg Donkey Anti-Goat IgG (H+L) ATTO490LS 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification removed donkey serum proteins and immunoglobulins not specifically binding to goat IgG. Following conjugation to ATTO490LS, the antibody was further purified by gel filtration to remove unconjugated dye and low-molecular-weight reaction components. Hypermol® secondary antibodies are highly specific and purified to remove unbound dye, minimizing background signal. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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