Donkey Anti-Goat IgG (H+L) ATTO514
Antibody produced in donkey, affinity purified
Cat. #: 2905-1MG
Applications
Suited for fluorescence microscopy, flow cytometry and other fluorescence-based applications. The preservative-free formulation minimizes interference in sensitive assay conditions.
Description
| Product name | Donkey Anti-Goat IgG (H+L) ATTO514 | DXG ATTO514 |
| Target species | Goat |
| Description |
ATTO-labeled secondary antibodies combine high fluorescence intensity with strong photostability and reliable performance in fluorescence-based detection. Donkey Anti-Goat IgG (H+L) ATTO514 is designed for the detection of goat primary antibodies and is suitable for fluorescence microscopy, flow cytometry and related fluorescence-based applications. The antibody is supplied without preservatives. After conjugation, free dye is removed by gel filtration to minimize background fluorescence and support reproducible staining performance. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2–5, Unbound dye ≤5% of total fluorescence. |
| Optical properties | λabs: 511 nm, λfl: 532 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is supplied as 1.0 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for reconstitution to 2 mg/ml. After reconstitution, the glycerol stock may be stored at -20°C without freezing. Avoid repeated freeze/thaw cycles. |
| Content | 1.0 mg Donkey Anti-Goat IgG (H+L) ATTO514 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification removed donkey serum proteins and immunoglobulins not specifically binding to goat IgG. Following conjugation to ATTO514, the antibody was further purified by gel filtration to remove unconjugated dye and low-molecular-weight reaction components. Hypermol® secondary antibodies are highly specific and purified to remove unbound dye, minimizing background signal. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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