Product name

Donkey Anti-Goat IgG ATTO514 (H+L)

Target species

Goat

Description

ATTO-labelled secondary antibodies belong to the new generation of fluorescent antibodies characterized by their exceptional fluorescence intensity and superior photostability. Donkey Anti-Goat ATTO514 (H+L) is specifically suited for high resolution microscopy, due to the high photostability and strong fluorescence quantum yield.

The antibody is suited for all immunobased applications, including live cell techniques, since it contains no preservatives.

Donkey Anti-Goat IgG ATTO514 (H+L) is an antigen-specific antibody. Affinity purification removed essentially all donkey serum proteins, including immunoglobulins not specifically binding to goat IgG. Donkey Anti-Goat IgG is conjugated to ATTO514 (Abs.max. 511 nm; Em.max. 533 nm) and further purified by gel filtration.
 
Donkey Anti-Goat IgG ATTO514 (H+L) is shipped in unit sizes of 1.0 mg as a lyophilized powder, shipped at room temperature. To reconstitute the antibody before use, a glycerol buffer is included for convenience. This allows to re-freeze the antibody after use at -20°C, to keep the quality and/or to prepare aliquots, which can be stored at -20°C and used in succession.

Specification

Donkey Anti-Goat IgG ATTO514 (H+L), Immunofluorescence: 1:500-1:1500, Degree of labeling (DOL) : 2-9, Unconjugated dye ≤5% of total fluorescence. 

Optical Properties

λex 511nm, λem 533nm

Form

Lyophilized

Nominal concentration

2 mg/ml (after reconstitution with buffer)

Content


Buffer

1.0 mg Donkey Anti-Goat IgG (H+L) ATTO514
1.0 ml Glycerol buffer (to reconstitute the lyophilized antibody)

50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4.

Clonality

Polyclonal

Isotype

IgG

Purification notes

Column affinity purified.

Storage instructions

Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

Shipped at ambient temperature.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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