Donkey Anti-Goat IgG (H+L) ATTO550
Antibody produced in donkey, affinity purified
Cat. #: 2907-1MG
Applications
Suited for fluorescence microscopy, flow cytometry and other fluorescence-based applications. The preservative-free formulation minimizes interference in sensitive assay conditions.
Description
| Product name | Donkey Anti-Goat IgG (H+L) ATTO550 | DXG ATTO550 |
| Target species | Goat |
| Description |
ATTO-labeled secondary antibodies combine high fluorescence intensity with strong photostability and reliable performance in fluorescence-based immunodetection. Donkey Anti-Goat IgG (H+L) ATTO550 specifically detects goat primary antibodies and is suitable for widefield, confocal and TIRF microscopy, flow cytometry, single-molecule detection and high-resolution fluorescence imaging. ATTO550 has an absorption maximum at 554 nm and a fluorescence maximum at 576 nm, producing orange fluorescence. The conjugate can be excited with 532 nm and 561 nm laser lines, with 561 nm providing closer spectral matching to the absorption maximum. Strong absorption, a high fluorescence quantum yield and pronounced photostability support sensitive detection, repeated scanning and prolonged image acquisition. ATTO550 is particularly well suited to single-molecule and high-resolution microscopy because of its low triplet-state formation and resistance to photobleaching. Donkey Anti-Goat IgG (H+L) ATTO550 is antigen-specific; affinity purification removes essentially all donkey serum proteins, including immunoglobulins not specifically binding to goat IgG. Following conjugation to ATTO550, the antibody is further purified by gel filtration to remove unbound dye and support low-background, reproducible fluorescence staining. The product is supplied without preservatives. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2–5, Unbound dye ≤5% of total fluorescence. |
| Optical properties | λabs: 554 nm, λfl: 576 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is supplied as 1.0 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for reconstitution to 2 mg/ml. After reconstitution, the glycerol stock may be stored at -20°C without freezing. Avoid repeated freeze/thaw cycles. |
| Content | 1.0 mg Donkey Anti-Goat IgG (H+L) ATTO550 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification removed donkey serum proteins and immunoglobulins not specifically binding to goat IgG. Following conjugation to ATTO550, the antibody was further purified by gel filtration to remove unconjugated dye and low-molecular-weight reaction components. Hypermol® secondary antibodies are highly specific and purified to remove unbound dye, minimizing background signal. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
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Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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