Donkey Anti-Guinea Pig IgG (H+L) ATTO430LS
Antibody produced in donkey, affinity purified
Cat. #: 3202-1MG
Applications
Suitable for immunofluorescence microscopy and related fluorescence-based assays. The conjugate is supplied without preservatives or protein additives such as BSA.
Description
| Product name | Donkey Anti-Guinea Pig IgG (H+L) ATTO430LS | DXGP ATTO430LS |
| Target species | Guinea Pig |
| Description |
ATTO-labeled secondary antibodies combine high fluorescence intensity with strong photostability and reliable performance in fluorescence-based immunodetection. Donkey Anti-Guinea Pig IgG (H+L) ATTO430LS specifically detects guinea pig primary antibodies and is suitable for widefield and confocal immunofluorescence microscopy as well as related fluorescence-based applications. ATTO430LS has an absorption maximum at 436 nm and a fluorescence maximum at 545 nm. Its exceptionally large Stokes shift of 109 nm provides pronounced spectral separation between excitation and emission, reduces excitation-light bleed-through into the detection channel and supports multiplex fluorescence experiments requiring clearly separated spectral windows. Appropriate excitation sources and emission filter sets are required for optimal detection. Donkey Anti-Guinea Pig IgG (H+L) ATTO430LS is antigen-specific; affinity purification removes essentially all donkey serum proteins, including immunoglobulins not specifically binding to guinea pig IgG. Following conjugation to ATTO430LS, the antibody is further purified by size-exclusion chromatography to remove unbound dye and low-molecular-weight reaction components. The conjugate is supplied without preservatives or protein additives such as BSA. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2–5, Unbound dye ≤5% of total fluorescence. |
| Optical properties | λabs: 436 nm, λfl: 545 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is supplied as 1.0 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for reconstitution to 2 mg/ml. After reconstitution, the glycerol stock may be stored at -20°C without freezing. Avoid repeated freeze/thaw cycles. |
| Content | 1.0 mg Donkey Anti-Guinea Pig IgG (H+L) ATTO430LS 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Column affinity purified. Following conjugation to ATTO430LS, the antibody is further purified by size-exclusion chromatography (SEC) to remove unbound dye and low-molecular-weight reaction components. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
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Nature. 2017;544(7651):465-470. doi:10.1038/nature22051.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016;7:10917. doi:10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016;113(32):8939-8944. doi:10.1073/pnas.1520428113.
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