Donkey Anti-Guinea Pig IgG (H+L) ATTO532
Antibody produced in donkey, affinity purified
Cat. #: 3206-1MG
Applications
Suitable for immunofluorescence microscopy and related fluorescence-based assays. The conjugate is supplied without preservatives or protein additives such as BSA.
Description
| Product name | Donkey Anti-Guinea Pig IgG (H+L) ATTO532 | DXGP ATTO532 |
| Target species | Guinea Pig |
| Description |
ATTO-labeled secondary antibodies combine high fluorescence intensity with strong photostability and reliable performance in fluorescence-based immunodetection. Donkey Anti-Guinea Pig IgG (H+L) ATTO532 specifically detects guinea pig primary antibodies and is suitable for widefield, confocal and TIRF microscopy, flow cytometry, single-molecule detection and super-resolution imaging, including PALM, dSTORM, SIM and STED. ATTO532 has an absorption maximum at 532 nm and a fluorescence maximum at 552 nm. The conjugate is efficiently excited within the 515–545 nm spectral range and is optimally matched to the 532 nm laser line. Its high fluorescence output and strong photostability support sensitive detection, repeated scanning and prolonged image acquisition in conventional, single-molecule and super-resolution fluorescence microscopy. Donkey Anti-Guinea Pig IgG (H+L) ATTO532 is antigen-specific; affinity purification removes essentially all donkey serum proteins, including immunoglobulins not specifically binding to guinea pig IgG. Following conjugation to ATTO532, the antibody is further purified by size-exclusion chromatography to remove unbound dye and low-molecular-weight reaction components. The conjugate is supplied without preservatives or protein additives such as BSA. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2–5, Unbound dye ≤5% of total fluorescence. |
| Optical properties | λabs: 532 nm, λfl: 552 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is supplied as 1.0 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for reconstitution to 2 mg/ml. After reconstitution, the glycerol stock may be stored at -20°C without freezing. Avoid repeated freeze/thaw cycles. |
| Content | 1.0 mg Donkey Anti-Guinea Pig IgG (H+L) ATTO532 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Column affinity purified. Following conjugation to ATTO532, the antibody is further purified by size-exclusion chromatography (SEC) to remove unbound dye and low-molecular-weight reaction components. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
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Nature. 2017;544(7651):465-470. doi:10.1038/nature22051.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
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Nat Commun. 2016;7:10917. doi:10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
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Proc Natl Acad Sci U S A. 2016;113(32):8939-8944. doi:10.1073/pnas.1520428113.
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