Donkey Anti-Guinea Pig IgG (H+L) ATTO647N
Antibody produced in donkey, affinity purified
Cat. #: 3210-1MG
Applications
Suited for immunofluorescence microscopy (recommended dilution 1:500–1:1500) and related fluorescence-based assays; preservative- and protein-additive-free (e.g., no BSA), supporting sensitive workflows and live-cell techniques.
Description
| Product name | Donkey Anti-Guinea Pig IgG (H+L) ATTO647N | DXGP ATTO647N |
| Target species | Guinea Pig |
| Description |
ATTO-labeled secondary antibodies are a new generation of fluorescent reagents characterized by high fluorescence intensity and superior photostability. Donkey Anti-Guinea Pig IgG (H+L) ATTO647N is antigen-specific; affinity purification effectively removes donkey serum proteins, including immunoglobulins not specifically binding to guinea pig IgG. The antibody is conjugated to ATTO647N and subsequently purified by size-exclusion chromatography (SEC). The fluorophore provides an excitation/emission profile of λex 647 nm and λem 669 nm, supporting multiplex imaging with clear spectral separation. Anti-Guinea Pig IgG ATTO647N is well suited for high-resolution microscopy due to its high photostability and strong fluorescence quantum yield. The conjugate is free of preservatives and protein additives (such as BSA), supporting sensitive fluorescence applications including live-cell techniques. The antibody is supplied as a lyophilized powder (1.0 mg) and shipped at room temperature. A glycerol buffer is provided for convenient reconstitution, enabling storage at -20°C and straightforward aliquoting for repeated use. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of labeling (DOL): 2–9, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 647 nm, λem: 669 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) |
| Content | 1.0 mg Donkey Anti-Guinea Pig IgG (H+L) ATTO647N 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Column affinity purified; following conjugation to ATTO647N, the antibody was further purified by SEC. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
| CAS no. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet 
Material and Safety Data Sheet
Table of Dyes and Light Sources
Catalogue: ATTO Secondary AntibodiesReferences
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
Product DataSheet
Material and Safety Data SheetTable of Dyes and Light Sources Catalogue: ATTO Secondary Antibodies
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
