Donkey Anti-Mouse IgG (H+L) ATTO490LS
Antibody produced in donkey, affinity purified
Cat. #: 2514-1MG
Applications
Suitable for immunofluorescence microscopy, multiplex fluorescence imaging and related fluorescence-based assays. The conjugate is supplied without preservatives or protein additives such as BSA.
Description
| Product name | Donkey Anti-Mouse IgG (H+L) ATTO490LS | DXM ATTO490LS |
| Target species | Mouse |
| Description |
ATTO-labeled secondary antibodies combine high fluorescence intensity with strong photostability and reliable performance in fluorescence-based immunodetection. Donkey Anti-Mouse IgG (H+L) ATTO490LS specifically detects mouse primary antibodies and is suitable for widefield and confocal immunofluorescence microscopy as well as multiplex fluorescence applications requiring pronounced spectral separation between excitation and emission. ATTO490LS has an absorption maximum at 496 nm and a fluorescence maximum at 661 nm, corresponding to an exceptionally large Stokes shift of 165 nm. The conjugate can be excited with the widely available 488 nm laser line while its fluorescence is detected in the far-red spectral range. The near-complete separation of excitation and emission reduces excitation-light bleed-through and enables multiplex combinations with green-emitting fluorophores such as ATTO488 or ATTO514. Donkey Anti-Mouse IgG (H+L) ATTO490LS is antigen-specific; affinity purification removes essentially all donkey serum proteins, including immunoglobulins not specifically binding to mouse IgG. Following conjugation to ATTO490LS, the antibody is further purified by size-exclusion chromatography to remove unbound dye and low-molecular-weight reaction components. The conjugate is supplied without preservatives or protein additives such as BSA. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2–5, Unbound dye ≤5% of total fluorescence. |
| Optical properties | λabs: 496 nm, λfl: 661 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is supplied as 1.0 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for reconstitution to 2 mg/ml. After reconstitution, the glycerol stock may be stored at -20°C without freezing. Avoid repeated freeze/thaw cycles. |
| Content | 1.0 mg Donkey Anti-Mouse IgG (H+L) ATTO490LS 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Column affinity purified. Following conjugation to ATTO490LS, the antibody is further purified by size-exclusion chromatography (SEC) to remove unbound dye and low-molecular-weight reaction components. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017;544(7651):465-470. doi:10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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