Donkey Anti-Mouse IgG ATTO514 (H+L)
Antibody produced in donkey, affinity purified
Cat. #: 2513-1MG
Applications
Ideal for fluorescence microscopy and live-cell imaging applications. Contains no preservatives for compatibility with sensitive techniques.
Description
| Product name | Donkey Anti-Mouse IgG ATTO514 (H+L) | DXM ATTO514 |
| Target species | Mouse |
| Description |
ATTO-labelled secondary antibodies belong to the new generation of fluorescent antibodies characterized by their exceptional fluorescence intensity and superior photostability. Donkey Anti-Mouse IgG ATTO514 (H+L) is optimized for high-resolution fluorescence microscopy, providing reliable and brilliant signals under demanding imaging conditions. ATTO514 exhibits a distinct excitation/emission spectrum (λex: 510 nm, λem: 530 nm), offering excellent spectral separation and compatibility with multiplex imaging setups. Its strong fluorescence signal supports sensitive detection and precise spatial resolution, including in complex biological samples. Donkey Anti-Mouse IgG ATTO514 (H+L) is an antigen-specific antibody and affinity purified to remove essentially all donkey serum proteins, including immunoglobulins not specifically binding to mouse IgG. The antibody is preservative-free and carrier-free (no BSA), minimizing non-specific background and supporting sensitive applications. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 3–7, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 510 nm, λem: 530 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) |
| Content | 1.0 mg Donkey Anti-Mouse IgG ATTO514 (H+L) 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purified; dye-conjugate further purified by gel filtration. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Material and Safety Data Sheet
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
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Product DataSheet
Material and Safety Data Sheet
Table of Dyes and Light Sources
Catalogue: ATTO Secondary Antibodies
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
