Donkey Anti-Mouse IgG (H+L) ATTO514
Antibody produced in donkey, affinity purified
Cat. #: 2515-1MG
Applications
Suitable for immunofluorescence microscopy, multiplex fluorescence imaging and related fluorescence-based assays. The conjugate is supplied without preservatives or protein additives such as BSA.
Description
| Product name | Donkey Anti-Mouse IgG (H+L) ATTO514 | DXM ATTO514 |
| Target species | Mouse |
| Description |
ATTO-labeled secondary antibodies combine strong fluorescence output with high photostability and reliable performance in fluorescence-based immunodetection. Donkey Anti-Mouse IgG (H+L) ATTO514 specifically detects mouse primary antibodies and is suitable for widefield, confocal and TIRF microscopy, flow cytometry, single-molecule detection and super-resolution imaging, including PALM, dSTORM and STED. ATTO514 has an absorption maximum at 511 nm and a fluorescence maximum at 532 nm, corresponding to a Stokes shift of 21 nm. The conjugate is efficiently excited in the 510–535 nm spectral range and is particularly well matched to the 514 nm argon-ion laser line. Its high fluorescence output and strong photostability support sensitive detection, repeated scanning and prolonged image acquisition in conventional, single-molecule and super-resolution fluorescence microscopy. Donkey Anti-Mouse IgG (H+L) ATTO514 is antigen-specific; affinity purification removes essentially all donkey serum proteins, including immunoglobulins not specifically binding to mouse IgG. Following conjugation to ATTO514, the antibody is further purified by size-exclusion chromatography to remove unconjugated dye and low-molecular-weight reaction components. The conjugate is supplied without preservatives or protein additives such as BSA. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2–5, Unconjugated dye ≤5% of total fluorescence. |
| Optical properties | λabs: 511 nm, λfl: 532 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is supplied as 1.0 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for reconstitution to 2 mg/ml. After reconstitution, the glycerol stock may be stored at -20°C without freezing. Avoid repeated freeze/thaw cycles. |
| Content | 1.0 mg Donkey Anti-Mouse IgG (H+L) ATTO514 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Column affinity purified. Following conjugation to ATTO514, the antibody is further purified by size-exclusion chromatography (SEC) to remove unconjugated dye and low-molecular-weight reaction components. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017;544(7651):465-470. doi:10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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