Donkey Anti-Mouse IgG ATTO532 (H+L)
Antibody produced in donkey, affinity purified
Cat. #: 2516-1MG
Applications
Ideal for fluorescence microscopy and live-cell imaging applications. Contains no preservatives for compatibility with sensitive techniques.
Description
| Product name | Donkey Anti-Mouse IgG ATTO532 (H+L) | DXM ATTO532 |
| Target species | Mouse |
| Description |
ATTO-labelled secondary antibodies belong to the new generation of fluorescent antibodies characterized by their exceptional fluorescence intensity and superior photostability. Donkey Anti-Mouse IgG ATTO532 (H+L) is specifically suited for high-resolution microscopy, due to the high photostability and strong fluorescence quantum yield. The antibody is preservative-free and therefore compatible with sensitive applications, including live cell techniques. ATTO532 provides a distinct excitation/emission spectrum (λex: 532 nm, λem: 553 nm) enabling robust signal generation and straightforward multiplex panel design. Donkey Anti-Mouse IgG ATTO532 (H+L) is an antigen-specific antibody. Affinity purification removed essentially all donkey serum proteins, including immunoglobulins not specifically binding to mouse IgG. Donkey Anti-Mouse IgG is conjugated to ATTO532 and further purified by gel filtration. Donkey Anti-Mouse IgG ATTO532 (H+L) is supplied in unit sizes of 1.0 mg as a lyophilized powder, shipped at room temperature. For convenient reconstitution, a glycerol buffer is provided. This enables re-freezing of the antibody at -20°C after use to maintain quality and allows for the preparation of aliquots, which can also be stored at -20°C for sequential use. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2–9, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 532 nm, λem: 553 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) |
| Content | 1.0 mg Donkey Anti-Mouse IgG ATTO532 (H+L) 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Column affinity purified; dye-conjugate further purified by gel filtration. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Properties
|
Form |
Lyophilized |
|
Nominal concentration |
2 mg/ml (after reconstitution with buffer) |
|
Content |
1.0 mg Donkey Anti-Mouse IgG (H+L) ATTO532 |
|
Clonality |
Polyclonal |
|
Isotype |
IgG |
|
Purification notes |
Column affinity purified. |
|
Storage instructions |
Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles. |
|
Shipping conditions |
Shipped at ambient temperature. |
|
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
|
CAS no. |
Further Information
Product DataSheet 
Material and Safety Data Sheet
Table of Dyes and Light Sources
Catalogue: ATTO Secondary AntibodiesReferences
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
Product DataSheet
Material and Safety Data SheetTable of Dyes and Light Sources Catalogue: ATTO Secondary Antibodies
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
