Donkey Anti-Mouse IgG (H+L) ATTO647N
Antibody produced in donkey, affinity purified
Cat. #: 2521-1MG
Applications
Suitable for immunofluorescence microscopy, multiplex fluorescence imaging and related fluorescence-based assays. The conjugate is supplied without preservatives or protein additives such as BSA.
Description
| Product name | Donkey Anti-Mouse IgG (H+L) ATTO647N | DXM ATTO647N |
| Target species | Mouse |
| Description |
ATTO-labeled secondary antibodies combine strong fluorescence output with high photostability and reliable performance in fluorescence-based immunodetection. Donkey Anti-Mouse IgG (H+L) ATTO647N specifically detects mouse primary antibodies and is suitable for widefield, confocal and TIRF microscopy, flow cytometry, single-molecule detection and super-resolution imaging, including PALM, dSTORM and STED. ATTO647N has an absorption maximum at 647 nm and a fluorescence maximum at 669 nm, corresponding to a Stokes shift of 22 nm. The conjugate is efficiently excited with 633 nm and 640 nm laser lines and is detected in the far-red spectral range. Its strong fluorescence output, high photostability and favorable far-red spectral characteristics support sensitive detection, repeated scanning and prolonged image acquisition in conventional, single-molecule and super-resolution fluorescence microscopy. Donkey Anti-Mouse IgG (H+L) ATTO647N is antigen-specific; affinity purification removes essentially all donkey serum proteins, including immunoglobulins not specifically binding to mouse IgG. Following conjugation to ATTO647N, the antibody is further purified by size-exclusion chromatography to remove unconjugated dye and low-molecular-weight reaction components. The conjugate is supplied without preservatives or protein additives such as BSA. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2–5, Unconjugated dye ≤5% of total fluorescence. |
| Optical properties | λabs: 647 nm, λfl: 669 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is supplied as 1.0 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for reconstitution to 2 mg/ml. After reconstitution, the glycerol stock may be stored at -20°C without freezing. Avoid repeated freeze/thaw cycles. |
| Content | 1.0 mg Donkey Anti-Mouse IgG (H+L) ATTO647N 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Column affinity purified. Following conjugation to ATTO647N, the antibody is further purified by size-exclusion chromatography (SEC) to remove unconjugated dye and low-molecular-weight reaction components. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
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Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017;544(7651):465-470. doi:10.1038/nature22051.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016;7:10917. doi:10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016;113(32):8939-8944. doi:10.1073/pnas.1520428113.
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