Donkey Anti-Mouse IgG (H+L) ATTO700
Antibody produced in donkey, affinity purified
Cat. #: 2522-1MG
Applications
Suitable for immunofluorescence microscopy, multiplex fluorescence imaging and related fluorescence-based assays. The conjugate is supplied without preservatives or protein additives such as BSA.
Description
| Product name | Donkey Anti-Mouse IgG (H+L) ATTO700 | DXM ATTO700 |
| Target species | Mouse |
| Description |
ATTO-labeled secondary antibodies combine strong fluorescence output with high photostability and reliable performance in fluorescence-based immunodetection. Donkey Anti-Mouse IgG (H+L) ATTO700 specifically detects mouse primary antibodies and is suitable for widefield and confocal fluorescence microscopy, flow cytometry, near-infrared fluorescence detection and multiplex imaging applications requiring a spectrally separated long-wavelength channel. ATTO700 has an absorption maximum at 700 nm and a fluorescence maximum at 719 nm, corresponding to a Stokes shift of 19 nm. The conjugate is best matched to excitation sources in the red to near-infrared spectral range, particularly laser lines near 690–700 nm. Its long-wavelength emission can reduce interference from sample autofluorescence and supports sensitive detection in complex specimens and multiplex fluorescence experiments. Donkey Anti-Mouse IgG (H+L) ATTO700 is antigen-specific; affinity purification removes essentially all donkey serum proteins, including immunoglobulins not specifically binding to mouse IgG. Following conjugation to ATTO700, the antibody is further purified by size-exclusion chromatography to remove unconjugated dye and low-molecular-weight reaction components. The conjugate is supplied without preservatives or protein additives such as BSA. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2–5, Unconjugated dye ≤5% of total fluorescence. |
| Optical properties | λabs: 700 nm, λfl: 719 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is supplied as 1.0 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for reconstitution to 2 mg/ml. After reconstitution, the glycerol stock may be stored at -20°C without freezing. Avoid repeated freeze/thaw cycles. |
| Content | 1.0 mg Donkey Anti-Mouse IgG (H+L) ATTO700 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Column affinity purified. Following conjugation to ATTO700, the antibody is further purified by size-exclusion chromatography (SEC) to remove unconjugated dye and low-molecular-weight reaction components. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017;544(7651):465-470. doi:10.1038/nature22051.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016;7:10917. doi:10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016;113(32):8939-8944. doi:10.1073/pnas.1520428113.
Product quality backed by the Hypermol® Satisfaction Guarantee.
