PlexPure® Donkey Anti-Rabbit IgG (H+L) ATTO390
Ultra cross-adsorbed donkey secondary antibody; preservative-free.
Cat. #: 4931-05MG
Applications
Suitable for immunofluorescence microscopy, multiplex fluorescence imaging and related fluorescence-based assays. The conjugate is supplied without preservatives or protein additives such as BSA.
Description
| Product name | PlexPure® Donkey Anti-Rabbit IgG (H+L) ATTO390 | DXR ATTO390 PlexPure® |
| Target species | Rabbit |
| Description |
PlexPure® Donkey Anti-Rabbit IgG (H+L) ATTO390 is an antigen-affinity-purified fluorescent secondary antibody for selective detection of rabbit primary antibodies in immunofluorescence, multiplex fluorescence imaging and related fluorescence-based assays. Following antigen-specific affinity purification using rabbit IgG, the antibody is ultra cross-adsorbed against purified immunoglobulins from ten non-target mammalian species: donkey, human, mouse, Syrian hamster, horse, goat, rat, guinea pig, bovine and sheep IgG, together with chicken IgY. This defined PlexPure® purification strategy is designed to reduce non-target species reactivity in complex multiplex antibody panels. ATTO390 has an absorption maximum at 390 nm and a fluorescence maximum at 476 nm, corresponding to a large Stokes shift of 86 nm. The broad spectral separation between absorption and fluorescence can facilitate channel discrimination in appropriately configured fluorescence and multiplex imaging systems. PlexPure® Donkey Anti-Rabbit IgG (H+L) ATTO390 is antigen-specific; affinity purification removes essentially all donkey serum proteins, including immunoglobulins not specifically binding to rabbit IgG. Following conjugation to ATTO390, the antibody is further purified by size-exclusion chromatography to remove unbound dye and low-molecular-weight reaction components. The conjugate is supplied without preservatives or protein additives such as BSA. |
| Specification | Immunofluorescence: 1:300–1:1000, Degree of Labeling (DOL): 2–5, Unbound dye ≤5% of total fluorescence. |
| Optical properties | λabs: 390 nm, λfl: 476 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is supplied as 0.5 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for reconstitution to 2 mg/ml. After reconstitution, the glycerol stock may be stored at -20°C without freezing. Avoid repeated freeze/thaw cycles. |
| Content | 0.5 mg PlexPure® Donkey Anti-Rabbit IgG (H+L) ATTO390 1.0 ml Glycerol buffer (add 0.25 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 1.0 mM sodium phosphate, 0.01 M sodium chloride, pH 7.4. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Antigen-affinity purified against rabbit IgG with reactivity to heavy and light chains. Ultra cross-adsorbed against purified donkey, human, mouse, Syrian hamster, horse, goat, rat, guinea pig, bovine and sheep IgG, together with chicken IgY. Following conjugation to ATTO390, the antibody is further purified by size-exclusion chromatography (SEC) to remove unbound dye and low-molecular-weight reaction components. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
References
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Nature. 2017;544(7651):465-470. doi:10.1038/nature22051.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016;7:10917. doi:10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016;113(32):8939-8944. doi:10.1073/pnas.1520428113.
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