Donkey Anti-Rabbit IgG (H+L) ATTO430LS
Antibody produced in donkey, affinity purified
Cat. #: 2912-1MG
Applications
Suitable for immunofluorescence microscopy, multiplex fluorescence imaging and related fluorescence-based assays. The conjugate is supplied without preservatives or protein additives such as BSA.
Description
| Product name | Donkey Anti-Rabbit IgG (H+L) ATTO430LS | DXR ATTO430LS |
| Target species | Rabbit |
| Description |
ATTO-labeled secondary antibodies are fluorescent reagents characterized by high fluorescence intensity and strong photostability. Donkey Anti-Rabbit IgG (H+L) ATTO430LS is suitable for immunofluorescence microscopy, multiplex fluorescence imaging and related fluorescence-based detection methods. ATTO430LS has an absorption maximum at 436 nm and a fluorescence maximum at 545 nm. The resulting large Stokes shift of 109 nm provides substantial separation between absorption and fluorescence and can facilitate spectral discrimination in appropriately configured multiplex imaging experiments. Donkey Anti-Rabbit IgG (H+L) ATTO430LS is antigen-specific; affinity purification removes essentially all donkey serum proteins, including immunoglobulins not specifically binding to rabbit IgG. The antibody is conjugated to ATTO430LS and further purified by gel filtration to remove unconjugated dye and low-molecular-weight reaction components. The conjugate is supplied without preservatives or protein additives such as BSA. Its preservative-free formulation supports sensitive fluorescence-based applications. It may also be used for live-cell surface staining when the primary antibody recognizes an accessible extracellular epitope and the assay conditions have been appropriately optimized. The antibody is supplied as 1.0 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for reconstitution to 2 mg/ml. After reconstitution, the glycerol stock may be stored at -20 °C. Avoid repeated freeze/thaw cycles. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2–5, Unconjugated dye ≤5% of total fluorescence. |
| Optical properties | λabs: 436 nm, λfl: 545 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is supplied as 1.0 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for reconstitution to 2 mg/ml. After reconstitution, the glycerol stock may be stored at -20°C without freezing. Avoid repeated freeze/thaw cycles. |
| Content | 1.0 mg Donkey Anti-Rabbit IgG (H+L) ATTO430LS 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Column affinity purified. Following conjugation to ATTO430LS, the antibody is further purified by size-exclusion chromatography (SEC) to remove unconjugated dye and low-molecular-weight reaction components. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
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Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017;544(7651):465-470. doi:10.1038/nature22051.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016;7:10917. doi:10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016;113(32):8939-8944. doi:10.1073/pnas.1520428113.
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