Donkey Anti-Rabbit IgG ATTO490LS (H+L)
Antibody produced in donkey, affinity purified
Cat. #: 2914-1MG
Applications
Designed for fluorescence-based methods including immunofluorescence microscopy and multiplex staining. Preservative-free, supporting sensitive workflows and live-cell techniques.
Description
| Product name | Donkey Anti-Rabbit IgG (H+L) ATTO490LS | DXR ATTO490LS |
| Target species | Rabbit |
| Description |
ATTO-labeled secondary antibodies are fluorescent reagents characterized by high fluorescence intensity and strong photostability. Donkey Anti-Rabbit IgG (H+L) ATTO490LS is optimized for fluorescence microscopy workflows where robust signal intensity and photostability are required. ATTO490LS features a large Stokes shift of 165 nm with distinct excitation/emission characteristics (λex: 496 nm; λem: 661 nm), supporting spectral separation in multiplex imaging setups. Donkey Anti-Rabbit IgG (H+L) ATTO490LS is antigen-specific; affinity purification removes essentially all donkey serum proteins, including immunoglobulins not specifically binding to rabbit IgG. The antibody is conjugated to ATTO490LS and further purified by gel filtration. This reagent is preservative-free and is supplied as a lyophilized powder (1.0 mg) shipped at room temperature. A glycerol buffer is provided for convenient reconstitution and subsequent storage at -20°C (including aliquoting for repeated use). |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 3–7, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 496 nm, λem: 661 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) Supplied as 1.0 mg lyophilized antibody; shipped at room temperature. A glycerol buffer is included to support reconstitution and enable storage at -20°C (including aliquoting). |
| Content | 1.0 mg Donkey Anti-Rabbit IgG (H+L) ATTO490LS 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Column affinity purified. After dye conjugation, the antibody is further purified by gel filtration. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Material and Safety Data Sheet
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
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Product DataSheet
Material and Safety Data Sheet
Table of Dyes and Light Sources
Catalogue: ATTO Secondary Antibodies
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, Walker LA, Zhao Y, Edwards EM, Michki NS, Cheng HPJ, Ghazzi M, Chen TY, Chen M, Roossien DH, Cai D.
Front Neural Circuits. 2021 Oct 20;15:732183.
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
