Donkey Anti-Rabbit IgG (H+L) ATTO550
Antibody produced in donkey, affinity purified
Cat. #: 2917-1MG
Applications
Suitable for immunofluorescence microscopy, high-resolution fluorescence imaging, multiplex staining, indirect fluorescence detection in flow cytometry and related fluorescence-based assays. The preservative-free formulation may also be used for live-cell surface staining when the primary antibody recognizes an accessible extracellular epitope.
Description
| Product name | Donkey Anti-Rabbit IgG (H+L) ATTO550 | DXR ATTO550 |
| Target species | Rabbit |
| Description |
ATTO-labeled secondary antibodies combine strong fluorescence output with high photostability and reliable performance in fluorescence-based detection. Donkey Anti-Rabbit IgG (H+L) ATTO550 is designed for the detection of rabbit primary antibodies in immunofluorescence microscopy, high-resolution fluorescence imaging, multiplex staining and related fluorescence-based assays. ATTO550 has an absorption maximum at 550 nm and a fluorescence maximum at 576 nm. The resulting Stokes shift is 26 nm and supports effective fluorescence detection and channel separation in appropriately configured imaging experiments. Donkey Anti-Rabbit IgG (H+L) ATTO550 is antigen-specific; affinity purification removes essentially all donkey serum proteins, including immunoglobulins not specifically binding to rabbit IgG. The antibody is conjugated to ATTO550 and further purified by gel filtration. The conjugate is supplied without preservatives or protein additives such as BSA. Gel filtration following dye conjugation removes unconjugated dye and low-molecular-weight reaction components. The preservative-free formulation supports sensitive fluorescence-based applications and may also be used for live-cell surface staining when the primary antibody recognizes an accessible extracellular epitope. The antibody is supplied as 1.0 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for reconstitution to 2 mg/ml. After reconstitution, the glycerol stock may be stored at -20°C. Avoid repeated freeze/thaw cycles. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2–5, Unconjugated dye ≤5% of total fluorescence. |
| Optical properties | λabs: 550 nm, λfl: 576 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is supplied as 1.0 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for reconstitution to 2 mg/ml. After reconstitution, the glycerol stock may be stored at -20°C without freezing. Avoid repeated freeze/thaw cycles. |
| Content | 1.0 mg Donkey Anti-Rabbit IgG (H+L) ATTO550 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Column affinity purified. Following conjugation to ATTO550, the antibody is further purified by size-exclusion chromatography (gel filtration) to remove unconjugated dye and low-molecular-weight reaction components. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017;544(7651):465-470. doi:10.1038/nature22051.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016;7:10917. doi:10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016;113(32):8939-8944. doi:10.1073/pnas.1520428113.
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