Product name

Donkey Anti-Rabbit IgG ATTO647N

Target species

Rabbit

Description

Donkey Anti-Rabbit IgG ATTO647N is specifically suited for high resolution microscopy, due to the high photostability and strong fluorescence quantum yield. 

It is thus specifically suited for all multiplex approaches. The antibody is used for all immunobased applications, including live cell techniques, since it contains no preservatives.

Donkey Anti-Rabbit IgG ATTO647N (H+L) is an antigen-specific antibody. Affinity purification removed essentially all donkey serum proteins, including immunoglobulins not specifically binding to Rabbit IgG. Donkey Anti-Rabbit IgG is conjugated to ATTO647N (Abs.max. 647 nm; Em.max. 669 nm) and further purified by gel filtration.
 
Donkey Anti-Rabbit IgG ATTO647N (H+L) is shipped in unit sizes of 1.0 mg as a lyophilized powder, shipped at room temperature. To reconstitute the antibody before use, a glycerol buffer is included for convenience. This allows to re-freeze the antibody after use at -20°C, to keep the quality and/or to prepare aliquots, which can be stored at -20°C and used in succession.

Specification

Donkey Anti-Rabbit IgG ATTO647N (H+L), Immunofluorescence: 1:500-1:1500, Degree of labeling (DOL) : 2-9, Unconjugated dye ≤5% of total fluorescence. 

Optical Properties

λex 647m, λem 669nm

Form

Lyophilized

Nominal concentration

2 mg/ml (after reconstitution with buffer)

Content



Buffer

1.0 mg Donkey Anti-Rabbit IgG (H+L) ATTO647N
1.0 ml Glycerol buffer (to reconstitute the lyophilized antibody)


50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4.

Clonality

Polyclonal

Isotype

IgG

Purification notes

Column affinity purified.

Storage instructions

Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

Shipped at ambient temperature.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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