Donkey Anti-Rat IgG (H+L) ATTO390
Antibody produced in donkey, affinity purified
Cat. #: 3401-1MG
Applications
Suitable for immunofluorescence microscopy, multiplex fluorescence imaging and related fluorescence-based assays. The conjugate is supplied without preservatives or protein additives such as BSA.
Description
| Product name | Donkey Anti-Rat IgG (H+L) ATTO390 | DXRT ATTO390 |
| Target species | Rat |
| Description |
ATTO-labeled secondary antibodies combine strong fluorescence output with high photostability and reliable performance in fluorescence-based detection. Donkey Anti-Rat IgG (H+L) ATTO390 is designed for the detection of rat primary antibodies in immunofluorescence microscopy, multiplex fluorescence imaging and related fluorescence-based assays. ATTO390 has an absorption maximum at 390 nm and a fluorescence maximum at 476 nm. The resulting large Stokes shift of 86 nm provides broad spectral separation between absorption and fluorescence and can facilitate channel discrimination in appropriately configured multiplex experiments. Donkey Anti-Rat IgG (H+L) ATTO390 is antigen-specific; affinity purification removes essentially all donkey serum proteins, including immunoglobulins not specifically binding to rat IgG. The antibody is conjugated to ATTO390 and further purified by gel filtration. The conjugate is supplied without preservatives or protein additives such as BSA. Following dye conjugation, the antibody is further purified by size-exclusion chromatography to remove unconjugated dye and low-molecular-weight reaction components. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2–5, Unconjugated dye ≤5% of total fluorescence. |
| Optical properties | λabs: 390 nm, λfl: 476 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is supplied as 1.0 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for reconstitution to 2 mg/ml. After reconstitution, the glycerol stock may be stored at -20°C without freezing. Avoid repeated freeze/thaw cycles. |
| Content | 1.0 mg Donkey Anti-Rat IgG (H+L) ATTO390 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Column affinity purified. Following conjugation to ATTO390, the antibody is further purified by size-exclusion chromatography (SEC) to remove unconjugated dye and low-molecular-weight reaction components. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017;544(7651):465-470. doi:10.1038/nature22051.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016;7:10917. doi:10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016;113(32):8939-8944. doi:10.1073/pnas.1520428113.
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