Donkey Anti-Rat IgG (H+L) ATTO488
Antibody produced in donkey, affinity purified
Cat. #: 3403-1MG
Applications
Suitable for immunofluorescence microscopy, high-resolution fluorescence imaging, multiplex staining, selected super-resolution microscopy workflows and indirect fluorescence detection in flow cytometry. The preservative-free, carrier-free formulation also supports sensitive assays and live-cell surface staining when the primary antibody recognizes an accessible extracellular epitope. The ATTO488 fluorophore may additionally be used in appropriately designed FRET systems.
Description
| Product name | Donkey Anti-Rat IgG (H+L) ATTO488 | DXRT ATTO488 |
| Target species | Rat |
| Description |
ATTO-labeled secondary antibodies combine high fluorescence intensity with strong photostability for reliable fluorescence-based detection. Donkey Anti-Rat IgG (H+L) ATTO488 is designed for the detection of rat primary antibodies and provides bright green fluorescence for conventional and high-resolution fluorescence microscopy, multiplex staining and selected super-resolution imaging workflows. The conjugate can also be used for indirect fluorescence detection in flow cytometry. ATTO488 has an absorption maximum at 501 nm and a fluorescence maximum at 523 nm. The corresponding Stokes shift is 22 nm. The spectral profile supports combination with appropriately separated fluorophores in multicolor experiments. The ATTO488 fluorophore may also be incorporated into suitably designed FRET systems, with performance depending on the selected partner fluorophore and assay geometry. Donkey Anti-Rat IgG (H+L) ATTO488 is antigen-specific; affinity purification removes essentially all donkey serum proteins, including immunoglobulins not specifically binding to rat IgG. The antibody is conjugated to ATTO488 and further purified by gel filtration. The conjugate is supplied without preservatives or protein additives such as BSA. For live-cell applications, it is suitable for surface staining when the primary antibody recognizes an extracellularly accessible epitope. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2–5, Unconjugated dye ≤5% of total fluorescence. |
| Optical properties | λabs: 501 nm, λfl: 523 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is supplied as 1.0 mg lyophilized powder and shipped at ambient temperature. A glycerol buffer is included for reconstitution to 2 mg/ml. After reconstitution, the glycerol stock may be stored at -20°C without freezing. Avoid repeated freeze/thaw cycles. |
| Content | 1.0 mg Donkey Anti-Rat IgG (H+L) ATTO488 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Column affinity purified. Following conjugation to ATTO488, the antibody is further purified by size-exclusion chromatography (SEC) to remove unconjugated dye and low-molecular-weight reaction components. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
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Nature. 2017;544(7651):465-470. doi:10.1038/nature22051.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
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Nat Commun. 2016;7:10917. doi:10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
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Proc Natl Acad Sci U S A. 2016;113(32):8939-8944. doi:10.1073/pnas.1520428113.
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