DTT (500mM)
Reliable, convenient and always fresh DTT solution.
Cat.#:5128-01
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Applications
Reagent for maintaining SH groups in the reduced state.
Description
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Product name |
DTT; Synonym: threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT |
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Product description |
An excellently suited reagent for maintaining SH groups in the reduced state to quantitatively reduce disulfide bonds. DTT is effective in SDS sample buffers for reducing protein disulfide bonds prior to SDS-PAGE and is a vital component of various buffers for cytoskeletal proteins. |
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Overview |
DTT is of low-odor, far more stable in solution and less toxic than 2-mercaptoethanol. Due to its strong reducing potential only a seven fold lower concentration of DTT (100 mM) is needed than for 2-mercaptoethanol (700 mM). |
Properties
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Form |
Lyophilized, ready-to-use. |
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Quantity |
2x 500mM DTT (sterile filtered before freeze-drying). Add 1.0ml of ultrapure H2O to each tube to make a fresh 500mM stock solution. |
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Formula |
C4H10O2S2; Molecular weight: 154.25 g/mol |
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Purity |
Ultrapure reagents. |
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Storage instructions |
Store at –20°C upon arrival. The product will be stable in performance for at least 1 year from the date of purchase. Solubilized DTT can be aliquoted and stored at -70°C for use within 3 months. Thawed aliquots should be used within 1 week. Avoid refreezing. |
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Shipping conditions |
At ambient temperature. Upon delivery store at -20°C. |
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Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
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CAS no. |
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