Actin-Trialkit F-Actin Binding (alpha skeletal muscle actin)

Product name

Actin

Target species

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Triallkit description

The most common use of actin is to analyze and characterize binding of ligands to F-actin. This is also especially useful for the characterization of recombinant proteins and fragments. The Trialkit manual describes how to make F-actin and how to perform functional tests to verify of the biological activity of actin binding proteins. Since a specific binding to actin filaments indicates the correct folding and structural integrity of the actin binding protein itself, the assays provided with this Trialkit are a valuable tool.

The Trialkit is available with 2x0.5mg rabbit skeletal muscle actin (Cat. # T8010-01) or bovine cardiac actin (Cat. # T8100-01). Buffers are included to maintain the biological activity of actin, and a Trialkit Manual introducing to the use of actin. We guarantee a stable performance of the kit components when stored at -70°C upon arrival for 6 months.. 

Purity

>99% by scanning densitometry.

Form

Lyophilized, ready-to-use.

Kit content

2x0.5 mg G-Actin (lyophilized powder);
1x50 ml MonoMix (lyophilized G-actin ATP-buffer);
1x1 ml PolyMix 10x stock (lyophilized F-actin ATP-buffer, pH 7.4);
1x Trialkit Manual F-Actin Binding;

Actin Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with ultrapure water to obtain a 1mg/ml solution.

Related products

For dilution of G-actin or exchange of ligand buffer into actin compatible buffer, MonoMix (Cat. #.: 5100-0*) may be used. In the absence of nucleators, the polymerization of G-actin is initiated by PolyMix (Cat.#: 5000-0*). 

Purification notes

Purified from rabbit skeletal muscle, GPC.

Protein concentration

Determined by the Biuret method.

Storage instructions

We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing.     

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

51005-14-2 (Actin)